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We narrowed to 32,143 results for: Eng

Showing: 5881 - 5900 of 32143 results

  3. Ai27

    Plasmid
    #34630
    Purpose
    Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.
    Insert
    Floxed hChR2(H134R)-tdTomato
    Use
    Mouse Targeting
    Tags
    tdTomato
    Expression
    Mutation
    His 134 has been mutated to Arg
    Promoter
    CAG
    Available since
    Feb. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    34630-plasmid-map-sequence-id-41505

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  4. pHRIG-PRAS40

    Plasmid
    #53599
    Purpose
    lentiviral expression of constitutively active PRAS40 and EGFP
    Depositor
    Heng Zhao
    Article
    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1
    Insert
    PRAS40 (AKT1S1 Human)
    Use
    Lentiviral
    Tags
    IRES-EGFP and myc
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    48227-plasmid-map-sequence-id-69517

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  8. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Expression
    Mutation
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  14. BE3-hA3A-R128A

    Plasmid
    #132944
    Purpose
    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-hA3A(R128A)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-hA3A(R128A)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132944-plasmid-map-sequence-id-314824

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  16. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80066
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80066-plasmid-map-sequence-id-152619

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  17. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80067
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80067-plasmid-map-sequence-id-152621

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  20. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80060
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80060-plasmid-map-sequence-id-152594

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Showing: 5881 - 5900 of 32143 results