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pQCXIZ GFP PODXL A WT

Plasmid

#202413

Purpose

Expression of GFP-tagged PODXL (isoform A) WT

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2945

Plasmid

#144421

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pZR060_CROPseq-sgCtrl-CRISPRi

Plasmid

#180269

Purpose

Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3CD

Plasmid

#174190

Purpose

Expresses PIK3CD in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3CD (PIK3CD Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL2-pCMV7.1

Plasmid

#155020

Purpose

N-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 2 (CUL2 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKate2-B_UTR

Plasmid

#68480

Purpose

Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKate2-B_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-epsilon-COP-GFP

Plasmid

#66604

Purpose

Expresses epsilon COP as a GFP fusion

Depositor

David Stephens

Article

Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

Insert

epsilon COP (COPE Bovine)

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-pri-let-7g

Plasmid

#51381

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7g (MIRLET7G Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pEMS1199

Plasmid

#29100

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple53 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH BRCC36

Plasmid

#27496

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

BRCC36 (BRCC3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pID3.01B

Plasmid

#22720

Depositor

Geraldine Seydoux

Article

D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52.

Insert

unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’

Expression

Bacterial

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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TFORF3015

Plasmid

#144491

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PPARA (PPARA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-SOX2

Plasmid

#130693

Purpose

Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human SOX2 (SOX2 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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MYC-NKX3.1

Plasmid

#42895

Depositor

Edward Gelmann

Article

Markowski et al Cancer Res. 2008 Sep 1;68(17):6896-901.

Insert

NKX3.1 (NKX3-1 Human)

Tags

MYC

Expression

Mammalian

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
MMTV-GAL4-SV40

Plasmid

#19255

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Insert

GAL4

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
ZE25 Stbm-pCS2+

Plasmid

#17067

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm (vangl2 Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

Viral Prep

#104495-PHPeB

Purpose

Ready-to-use AAV PHP.eB particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Promoter

CAG

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only
pS648∙CsR

Plasmid

#197860

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Gm resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RhoH

Plasmid

#23230

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoH (RHOH Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only