We narrowed to 15,991 results for: LEA

1. Nanog-CreER targeting construct

   Plasmid

   #59720

   Purpose

   Cre-ER knockin targeting construct for mouse endogenous Nanog locus

   Depositor

   Jacob Hanna

   Article

   Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

   Insert

   creERT2
   

   Use

   Mouse Targeting

   Promoter

   mNanog

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC-NF8-d1-eGFP

   Plasmid

   #122036

   Purpose

   AAV vector, NFkappaB-dependent eGFP expression

   Depositor

   Liliane Tenenbaum

   Article

   Chtarto et al PLoS One. 2013;8(1):e53156. doi: 10.1371

   Insert

   eGFP
   

   Use

   AAV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHJ425

   Plasmid

   #20683

   Depositor

   Stephen Shaw

   Article

   Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

   Insert

   Ezrin (EZR Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Lys 253, 254, 262, 263 to Asn; and change…

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pIS1 DICER1 long-mut-let-7

   Plasmid

   #21651

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant let-7 microRNA binding sites

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

   Plasmid

   #112211

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   C-terminus of dCas9
   

   Use

   AAV

   Mutation

   D10A

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NG1237 pCI-TPI-PTC160-4H

   Plasmid

   #65804

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Tags

   4H (4 copies of short beta-globin 3'UTR for …

   Expression

   Mammalian

   Mutation

   premature stop codon at 160

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC94

   Plasmid

   #118912

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and eGFP-HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MDH1-miR-222-PGK-GFP-Blast

   Plasmid

   #31522

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-222 (Mir222 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pMal-SLFN2

   Plasmid

   #174322

   Purpose

   Bacterial expression of MBP-tagged mouse SLFN2

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   SLFN2 (Slfn2 Mouse)
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. VRER variant

    Plasmid

    #138561

    Purpose

    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. IBMc326

    Plasmid

    #161949

    Purpose

    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIS1 DICER1 long-mut-all

    Plasmid

    #21653

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated let-7 and miR103/107 microRNA binding sit…

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIS1 DICER1 long-mut-miR103/107

    Plasmid

    #21652

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant miR103/107 microRNA binding sites

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV Tmem53-V5

    Plasmid

    #175142

    Purpose

    Lentiviral expression of mouse Tmem53-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmem53 (Tmem53 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTox-EGFPsite1_NGA-PAM (BPK754)

    Plasmid

    #181747

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
    

    Use

    Selection plasmid

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL3

    Plasmid

    #184131

    Purpose

    For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Pges-1 (3.3kb)

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HOXC4 (human) MSCV

    Plasmid

    #8546

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXC4 (HOXC4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Contains improved ribosomal binding site and NcoI…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

    Plasmid

    #134866

    Purpose

    Expresses EGFP-tagged mutant of VAMP1

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Vesicle-associated membrane protein 1 (VAMP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 97-118

    Promoter

    EF1a

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-CUL1 L756A/I757A

    Plasmid

    #19938

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 L756A/I757A (CUL1 Human)
    

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutation of two adjacent hydrophobic residues, L…

    Available Since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

20. XE223 human myc-Chibby-pCS2+MT

    Plasmid

    #16825

    Depositor

    Randall Moon

    Article

    Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

    Insert

    myc-Chibby (CBY1 Human)
    

    Use

    Xenopus expression

    Tags

    6-myc

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only