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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,644 results for: eng

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Showing: 5881 - 5900 of 26644 results

  1. pCH61 (multiAsCas12a-KRAB piggyBac)

    Plasmid
    #217332
    Purpose
    piggyBac expression of multiAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Publication
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    multiAsCas12a
    Use
    CRISPR
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mammalian
    Mutation
    R1226A/E174R/S542R/K548R
    Promoter
    CAG
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217332-plasmid-map-sequence-id-426476

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  6. pCDNA3.1-H-Luc_cyto

    Plasmid
    #113929
    Purpose
    Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET
    Depositor
    Kai Johnsson
    Publication
    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H-Luc
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113929-plasmid-map-sequence-id-222526

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  7. pEF005-sgRNA-shuffle-in

    Plasmid
    #104441
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.
    Depositor
    Detlef Weigel
    Publication
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104441-plasmid-map-sequence-id-201145

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  9. GLI2 his

    Plasmid
    #87097
    Purpose
    expresses GLI2 in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GLI2 (GLI2 Human)
    Tags
    6x His, T7 tag, and Xpress tag
    Expression
    Mammalian
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87097-plasmid-map-sequence-id-201477

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  10. pcDNA3.1-ERBB3-V5/HIS

    Plasmid
    #201104
    Purpose
    expression of human ERBB3 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human ERBB3 receptor tyrosine kinase, full length, wildtype (ERBB3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201104-plasmid-map-sequence-id-394500

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  11. pcDNA3.1-FGFR2c-V5/HIS

    Plasmid
    #201107
    Purpose
    expression of human FGFR2 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201107-plasmid-map-sequence-id-394499

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  12. pBBR_Cas9_sp1

    Plasmid
    #154851
    Purpose
    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Spcas9 gene
    sgRNA scaffold
    Use
    CRISPR
    Tags
    6xHis
    Mutation
    Codon harmonized variant for expression in Rhodob…
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154851-plasmid-map-sequence-id-309592

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  16. gag-TadCBEd

    Plasmid
    #225144
    Purpose
    Packaging plasmid for TadCBEd in v3 PE-eVLP architecture
    Depositor
    David Liu
    Publication
    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-TadCBEd
    Expression
    Mammalian
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    225144-plasmid-map-sequence-id-445269

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  19. gGBEv6.3-SpG

    Plasmid
    #202630
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpG(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    hU6, EF1a, CBh
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202630-plasmid-map-sequence-id-398429

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  20. scFLARE1-p2A-eGFP

    Plasmid
    #158699
    Purpose
    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
    Depositor
    Alice Ting
    Publication
    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p
    Insert
    scFLARE1-p2A-eGFP
    Use
    AAV
    Tags
    Flag, V5. eGFP
    Expression
    Mammalian
    Mutation
    Truncated form of TEV protease (220-242 amino aci…
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158699-plasmid-map-sequence-id-315073

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Showing: 5881 - 5900 of 26644 results