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pWT057g

Plasmid

#96874

Purpose

HHR-bsgRNA-HEK4

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

HHR-bsgRNA-HEK4

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPKC1 (JCE575)

Plasmid

#89773

Purpose

yeast expression of PKC1

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC1 (PKC1 Budding Yeast)

Expression

Yeast

Promoter

yeast PKC

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pKF296

Plasmid

#74773

Purpose

template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV- 2xV5 tag (short version)

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_52

Plasmid

#63808

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_2

Plasmid

#63803

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_37

Plasmid

#63807

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAD01 S. cerevisiae telomerase RNA KBS

Plasmid

#61890

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)R61E

Plasmid

#60353

Purpose

R61E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R61E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E/R59E/R61E

Plasmid

#60355

Purpose

R53E R59E R61E triple mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E, R59E and R61E mutations; contains amino aci…

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab21 WT

Plasmid

#58455

Purpose

expression of drosophila YFP Rab21

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab21 (Rab21 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab6 WT

Plasmid

#58447

Purpose

expression of drosophila YFP-Rab6

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab2 WT

Plasmid

#58444

Purpose

expression of drosophila YFP-Rab2

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab2 (Rab2 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pAS-1 (perf)

Plasmid

#40836

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–perf

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAS-8 (seed1-8)

Plasmid

#40843

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–seed1-8

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
EGFP-hPMCA4b delta6

Plasmid

#47590

Depositor

Emanuel Strehler

Article

Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

Insert

PMCA4bdelta6 (ATP2B4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Aug. 26, 2013

Availability

Academic Institutions and Nonprofits only
pJDJRSF05

Plasmid

#46379

Depositor

Hideo Iwai

Article

Heinamaki et al Biomol NMR Assign. 2009 Jun;3(1):41-3. d

Insert

NpuDnaE intein inactive

Tags

H6-Smt3

Expression

Bacterial

Mutation

C1A

Promoter

T7

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-L3S2P36

Plasmid

#46007

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P36 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 2*

Plasmid

#32388

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

2nd E2F binding site mutated: GTC-->AAA

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only