We narrowed to 9,753 results for: try

1. plk1 T210D

   Plasmid

   #132964

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 (PLK1 Human)
   

   Expression

   Mammalian

   Mutation

   T210D

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. plk1 T210A

   Plasmid

   #132965

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 T210A (PLK1 Human)
   

   Expression

   Mammalian

   Mutation

   T210A

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-mVenus-CAAX

   Plasmid

   #75153

   Purpose

   Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mVenus-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+ GFP-2xESP3I-IRES-mCherry

   Plasmid

   #230996

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Type

   Empty backbone

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFP.E227

   Plasmid

   #138249

   Purpose

   Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   mCherry
   Terminator
   mTagBFP
   Terminator
   gfp
   Terminator
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P17_up1691, P20_992, and iP16_3622

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR59.4

   Plasmid

   #63175

   Purpose

   Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   OmpR (ompR )
   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2R3z-BASI-ccdB-BSAI

   Plasmid

   #118389

   Purpose

   Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   ccdB
   

   Use

   Unspecified

   Mutation

   See Depositor Comments Section

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p221z-CAS9p-TagRFP-t35s

   Plasmid

   #118386

   Purpose

   Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p-TagRFP
   

   Use

   CRISPR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Gal80ts

   Plasmid

   #51619

   Purpose

   vertebrate expression of full-length temperature-sensitive Gal80 with stop

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80ts (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Mutation

   temperature sensitive (G203V, G323R)*

   Available Since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENT-L1-Kozak-lacZ-stop-L2

    Plasmid

    #186359

    Purpose

    Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli, Synthetic)
    

    Use

    Expression of lacz reporter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-M2-TLR2

    Plasmid

    #12291

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I and H202Y; signal peptide (aa1-23) replaced …

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pYPQ132-tRNA2.0

    Plasmid

    #158394

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Without promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLG327

    Plasmid

    #128797

    Purpose

    Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pxyl(s)

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-FRT-KanR-FRT pA

    Plasmid

    #82601

    Purpose

    3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRT-Kan-FRT
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBW101ParsR-gfp

    Plasmid

    #78636

    Purpose

    pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J101-rbs32-arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Promoter

    see insert

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

18. p5E_unc503

    Plasmid

    #64020

    Purpose

    5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

    Depositor

    Peter Currie, Joachim Berger

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
    

    Use

    Gateway

    Promoter

    503-bp promoter of zebrafish unc-45b

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSW454 - pUFO

    Plasmid

    #115978

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pUFO
    

    Use

    Unspecified

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-NICD1

    Plasmid

    #46048

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Entry vector

    Tags

    Myc tag

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only