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We narrowed to 12,282 results for: LEA

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Showing: 5921 - 5940 of 12282 results

  1. pGBW-m4046268

    Plasmid
    #145423
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145423-plasmid-map-sequence-id-298350

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  2. pGBW-m4046332

    Plasmid
    #145413
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145413-plasmid-map-sequence-id-298850

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  3. pGBW-m4046437

    Plasmid
    #145415
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145415-plasmid-map-sequence-id-298868

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  4. pCEP4-CH115gp160

    Plasmid
    #123280
    Purpose
    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (CH115) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123280-plasmid-map-sequence-id-235246

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  5. pCEP4-25711gp160

    Plasmid
    #111841
    Purpose
    Mammalian expression plasmid for Env from the 25711 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (25711.2) Env
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111841-plasmid-map-sequence-id-210185

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  12. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Publication
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Available since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

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  13. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

    Plasmid
    #228721
    Purpose
    Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228721-plasmid-map-sequence-id-454005

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  14. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220610
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220610-plasmid-map-sequence-id-441133

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  15. pLV-REPORT(PGK)

    Plasmid
    #172328
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172328-plasmid-map-sequence-id-354688

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  16. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

    Plasmid
    #196885
    Purpose
    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    196885-plasmid-map-sequence-id-391918

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  17. pBactin-mOrange2-NEDD1-gTBD

    Plasmid
    #196858
    Purpose
    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mOrange2-N-gTBD (NEDD1 Human)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    196858-plasmid-map-sequence-id-391826

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  18. AV15_pCAG.Cas9.gRNA.S1

    Plasmid
    #199259
    Purpose
    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199259-plasmid-map-sequence-id-391662

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Showing: 5921 - 5940 of 12282 results