We narrowed to 15,033 results for: invs

1. Myog'-2A-mCherryNLS-PGK-Purodtk

   Plasmid

   #69547

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm (Myog Mouse)
   Myogenin 3' homology arm (Myog Mouse)
   Puro-delta-tk
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Expression

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Promoter

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-APC11

   Plasmid

   #19898

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   APC11 (ANAPC11 Human)
   

   Use

   Tags

   HA and T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pGas-miRFP670nano3

   Plasmid

   #184672

   Purpose

   G protein alpha-subunit internally tagged with miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   G protein alpha-s-miRFP670nano3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-NES-ZapCmR1.1

   Plasmid

   #59014

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. HGXPRT

   Plasmid

   #64201

   Purpose

   Expression of Plasmodium falciparum HGXPRT

   Depositor

   Vern Schramm

   Article

   Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

   Insert

   Hypoxanthine guanine xanthine phosphoribosyltransferase
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Nano-lantern(Ca2+)_117/pcDNA3

   Plasmid

   #51985

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-2GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. HA PRAS40 S183A

   Plasmid

   #15674

   Depositor

   John Lawrence

   Article

   Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

   Insert

   PRAS40 S183A (AKT1S1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S183A

   Promoter

   Available Since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

8. 2xFRB-myc

   Plasmid

   #20227

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   2xFRB (MTOR Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   1 FRB copy has a T2098L point mutation

   Promoter

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pFB-Flag-MyoD

   Plasmid

   #25994

   Depositor

   Jeffrey Dilworth

   Article

   Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

   Insert

   MyoD (Myod1 Mouse)
   

   Use

   Baculovirus transfer vector

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pME-QUAS5x:GFPNLS-SV40pA

    Plasmid

    #155115

    Purpose

    Middle entry vector containing QUAS5x element upstream of GFP-NLS.

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUAS5x:GFPNLS
    

    Use

    Gateway middle entry vector

    Tags

    Expression

    Mutation

    Promoter

    QUAS5x-E1b

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

    Plasmid

    #168189

    Purpose

    HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDM8-GlyCAM-1/IgG

    Plasmid

    #17841

    Depositor

    Steven Rosen

    Article

    Bistrup et al J Cell Biol. 1999 May 17. 145(4):899-910

    Insert

    GlyCAM-1/IgG (Glycam1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 25, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCFJ907

    Plasmid

    #44473

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, [4-3] Gateway
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-R4R3-3xmEGFP-C

    Plasmid

    #113751

    Purpose

    Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP-mEGFP-mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmEGFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. C-Ag15

    Plasmid

    #11868

    Depositor

    Martin Smith

    Article

    Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

    Insert

    Agrn (Agrn Mouse)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pCRII-HSV2.3 TCR beta chain

    Plasmid

    #74077

    Purpose

    gBT-I TCR beta chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR beta cDNA clone HSV2.3 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 Glt1a

    Plasmid

    #98705

    Purpose

    mammalian expression of Glt1a

    Depositor

    Paul Rosenberg

    Article

    Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

    Insert

    Glt1a (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I288V (please see depositor comments below)

    Promoter

    CMV

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. C-Ag20(0)

    Plasmid

    #11866

    Depositor

    Martin Smith

    Article

    Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

    Insert

    Agrn (Agrn Mouse)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

19. IO201: pMVP (R4-R3) mCherry

    Plasmid

    #121732

    Purpose

    pMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. tau exon 9–12 FTDP-17 N279K splicing minigene

    Plasmid

    #122967

    Purpose

    expresses circular tau RNAs with the FTDP-17 N279K

    Depositor

    Stefan Stamm

    Article

    Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

    Insert

    maps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FTDP-17 mutation

    Promoter

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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