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pVKG1-Puro

Plasmid

#108670

Purpose

Puromycin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

PURO

Use

CRISPR and Synthetic Biology

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Expression

Mammalian

Promoter

Cyclophilin A promoter

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0761

Plasmid

#133494

Purpose

Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-POL30

Plasmid

#99542

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB8786

Plasmid

#126920

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Golgi-targeted optodPLD

Plasmid

#140057

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pK3FS

Plasmid

#85776

Purpose

N-ICE plasmid pK3FS

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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YEp112-CUP1-His-Smt3

Plasmid

#99537

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNRB150

Plasmid

#36451

Depositor

Douglas Bishop

Article

Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

Insert

DMC1 (DMC1 Budding Yeast)

Tags

6His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pI6-MTU-DO-URA

Plasmid

#160030

Purpose

Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-URA3-3'SEC13-GFP

Plasmid

#25445

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

Insert

Sec13-Green Fluorescent Protein

Expression

Yeast

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Hyg)

Plasmid

#110082

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSBY2_ligD(FnCpf1)

Plasmid

#104623

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

crRNA to ligD

Use

CRISPR

Expression

Bacterial

Promoter

Pj23119

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAV0327

Plasmid

#133488

Purpose

Expression of nucleo-cytoplasmic sfGFP

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTCW007

Plasmid

#83525

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha1

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAV0478

Plasmid

#133498

Purpose

Expression of sfGFP targeted to the nucleus with two NLS signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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PHBA02

Plasmid

#83536

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Endosome-targeted optodPLD

Plasmid

#140055

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

2xFYVE, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-HIS

Plasmid

#160142

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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