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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Bin1b

Plasmid

#109509

Purpose

Muscle specific zebrafish expression of mKate2 fused to Bin1b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Bin1b (bin1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-tomato-TSC2-sgRNA

Plasmid

#196195

Purpose

Editing human TSC2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

TSC2 (TSC2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pY017 (pcDNA3.1-hPcCpf1)

Plasmid

#69989

Purpose

Expresses humanized PcCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPcCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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AA345

Plasmid

#216031

Purpose

Fragmid fragment: (Cas protein) Cas13d

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas13d_v1.1 [Dj]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_834

Plasmid

#216087

Purpose

Cas9 [Sp] knockout targeting CD59, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD59 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-6085x

Plasmid

#108653

Purpose

A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

PicchuEV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd2-1D4

Plasmid

#42264

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 2 (Fzd2 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd4-1D4

Plasmid

#42266

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 4 (Fzd4 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-FAPP1

Plasmid

#109503

Purpose

Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-FAPP1

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-2xFYVEhgs

Plasmid

#109505

Purpose

Muscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-2xFYVEhgs

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJT11

Plasmid

#98308

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

TRPL3ClLIS1>TRPL41B

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pPMVAd36

Plasmid

#98302

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

Expression

Yeast

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP-Lysosomes-20

Plasmid

#56228

Purpose

Localization: Lysosome Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LAMP1

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
actc1b-mKate2-rab32a

Plasmid

#109621

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab32a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32a (rab32a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOME0180

Plasmid

#178402

Purpose

Expresses KSHV ORF50 hyp tagged

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF 50

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pZ005 (AacC2c1 human codon optimized protein)

Plasmid

#70171

Purpose

Expresses AacC2c1 human codon optimized protein

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 protein

Tags

NLS

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab14

Plasmid

#109600

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab14

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab14 (rab14 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-NpgA

Plasmid

#126731

Purpose

yeast plasmid overexpressing NpgA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

npgA

Tags

6xHis

Expression

Yeast

Mutation

G951A,C957A

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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