We narrowed to 166,460 results for: Des

Showing: 5961 - 5980 of 166460 results

Results per page:

Sort results by:

pEasyG2_zeo

Plasmid

#184913

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEasyG2_nat

Plasmid

#184914

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEasyG3_zeo

Plasmid

#184917

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
enAsABE8e-dimer

Plasmid

#138507

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEG R2-iNeo-L3 #BGV207

Plasmid

#48986

Purpose

Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Insert

neomycin resistance

Use

Gateway Cloning

Tags

IRES (weak)

Promoter

NA

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAav-MCS-PQS1-3xFLAG

Plasmid

#84883

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

Multicloning sites and selection cassette

Use

AAV

Promoter

PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-blast-cfTuba_2

Plasmid

#26718

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Tuba_2 RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pAN1735

Plasmid

#127204

Purpose

Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1

Use

Synthetic Biology

Available Since

June 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p21 promoter D

Plasmid

#16465

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pENTR-ATXN1L

Plasmid

#16167

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Similar to Ataxin1 (ATXN1L Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p21 promoter F

Plasmid

#16467

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-ODC2

Plasmid

#133257

Purpose

Express FLAG-tagged ODC2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

ODC2 (ODC2 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs_FLAG-NDT2

Plasmid

#133255

Purpose

Express FLAG-tagged NDT2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NDT2 (YEA6 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-ASXL1-HARE-HTH domain

Plasmid

#107164

Purpose

Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

ASXL1 (Asxl1 Mouse)

Tags

Flag

Expression

Bacterial

Mutation

Contains amino acids 2-83

Promoter

T7

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK4-luciferase active (880)

Plasmid

#86657

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

867 base pairs including up to coding nucleotide …

Promoter

CDK5

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
4E2H.210_LUMABS

Plasmid

#155209

Purpose

Expresses 4E2H.210_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN1779

Plasmid

#127165

Purpose

Vector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)

Depositor

Hana El-Samad

Article

Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1

Use

Synthetic Biology

Available Since

Aug. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge