We narrowed to 7,663 results for: ski

1. pJET1.2_Bod1l_Anti-Sense

   Plasmid

   #124444

   Purpose

   Plasmid for anti-sense in situ probe in vitro transcription

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Insert

   Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)
   

   Use

   In situ probe

   Promoter

   T7

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET1.2_Gli3_Sense

   Plasmid

   #124439

   Purpose

   Plasmid for sense in situ probe in vitro transcription

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Insert

   GLI-Kruppel family member GLI3 (Gli3 Mouse)
   

   Use

   In situ probe

   Promoter

   T7

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-TRC.mKO2_shHRASLS.2

   Plasmid

   #110324

   Purpose

   TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   HRASLS (PLAAT1 Human, Homo sapiens)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-TRC.mKO2_shHRASLS.1

   Plasmid

   #110323

   Purpose

   TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   HRASLS (PLAAT1 Human, Homo sapiens)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP Kind2 S159/181/666E

   Plasmid

   #105314

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666E

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_ZNF597_WT

   Plasmid

   #82894

   Purpose

   Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   ZNF597 (ZNF597 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

   Plasmid

   #216248

   Purpose

   HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human RANGAP1 (RANGAP1 Human)
   

   Use

   CRISPR and TALEN; Hdr template

   Tags

   miniIAA7.3xFlag.P2A.BSD

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

   Plasmid

   #185910

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTight-9-124-BclxL

    Plasmid

    #60857

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTight

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid

    #205703

    Purpose

    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.2 TDP-43 YFP

    Plasmid

    #84911

    Purpose

    expression of TDP-43 YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-Cyclin A2

    Plasmid

    #215048

    Purpose

    Expresses EGFP-Cyclin A2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNA2 (CCNA2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Cyclin E1

    Plasmid

    #215053

    Purpose

    Expresses EGFP-Cyclin E1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.iAChSnFR

    Plasmid

    #137955

    Purpose

    Expresses iAChSnFR under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Has Service

    AAV1 and AAV9

    Insert

    iAChSnFR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2-HuC(elavl3)-CaMPARI2

    Plasmid

    #137185

    Purpose

    pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
    

    Use

    Tol2 plasmid for zebrafish

    Tags

    FLAG, HA, myc

    Promoter

    HuC(elavl3)

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304-KDM6A

    Plasmid

    #203580

    Purpose

    Expresses wild-type KDM6A in mammalian cells.

    Depositor

    Abhishek Chakraborty, William Kaelin

    Article

    Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

    Insert

    KDM6A (KDM6A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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