We narrowed to 451 results for: DHFR

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pENTR1A DHFR.cHSF1

Plasmid

#134737

Purpose

Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Gateway Cloning

Tags

DHFR

Mutation

Deletion of amino acids 186-202

Promoter

None

Available Since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DHFR.cHSF1

Plasmid

#134738

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available Since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31VDHFR_WTTS

Plasmid

#91227

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

Plasmid

#101109

Purpose

Encodes dCas9(N)-DHFR fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)-DHFR

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pERB217

Plasmid

#61500

Purpose

Const. Halotag-GFP-Mito + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Mito
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA C12 ecDHFR DD YFP HA

Plasmid

#192815

Purpose

Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

Depositor

John Hulleman

Article

Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

Insert

E. coli dihydrofolate reductase

Tags

enhanced yellow fluorescent protein and hemagglut…

Expression

Mammalian

Mutation

W74R/T113S/E120D/Q146L

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Atp5-DHFR-TEV (integrating)

Plasmid

#247219

Purpose

Plasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Atp5 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Oxa1-DHFR-TEV (integrating)

Plasmid

#247220

Purpose

Plasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Oxa1 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DD-T6B-WT-YFP

Plasmid

#235143

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Promoter

hSyn

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pERB215

Plasmid

#61499

Purpose

Const. Halotag-GFP-Nuf2 + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Nuf2
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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Rasgrf2-2A-dCre targeting vector

Plasmid

#61573

Purpose

Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Rasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)

Use

Cre/Lox and Mouse Targeting

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pDBD-M

Plasmid

#50572

Purpose

Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

Depositor

Alexander Varshavsky

Article

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pPL5618_pUG_MtxR

Plasmid

#60929

Purpose

PCR template vector for generating methotrexate resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Dihydrofolate reductase (DHFR Human)

Use

Loxp flanked deletion cassette template

Mutation

L22F F31S

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-G51PEKN

Plasmid

#118582

Purpose

Bacterial expression of E.coli DHFR G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

G51 mutated to P51; Glu-Lys-Asn residues inserted…

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDBD-R

Plasmid

#50571

Purpose

Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572

Depositor

Alexander Varshavsky

Article

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin

Expression

Yeast

Mutation

M1R (B-gal)

Promoter

Gal1

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
pDonorTp

Plasmid

#211788

Purpose

pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

dhfr

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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