We narrowed to 795 results for: KO

1. Lmajor_gp63_10_0460_P460G_D463N_S465A

   Plasmid

   #171645

   Purpose

   Expression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cells

   Depositor

   Dennis Ko

   Article

   Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

   Insert

   LmjF.10.0460 P460G D463N S465A
   

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   P460G; D463N; S465A

   Promoter

   Available since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Flag-stm2585

   Plasmid

   #174397

   Purpose

   Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWSK129-stm2585-gp130

   Plasmid

   #174404

   Purpose

   Bacterial expression of sarA-gp130 chimera under native promoter.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA-IL6ST chimera
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Endogenous

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWSK129-stm2585_S130A

   Plasmid

   #174403

   Purpose

   Bacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sarA with S130A mutation

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWSK129-stm2585_Y167F

   Plasmid

   #174402

   Purpose

   Bacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQ

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sarA with Y167F mutation

   Promoter

   Endogenous

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-Flag-stm2585_L139*

   Plasmid

   #174400

   Purpose

   S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   L139* (Truncation after 139th amino acid in sarA)

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-Flag-stm2585_S130A

   Plasmid

   #174399

   Purpose

   Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   S130A

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-Flag-stm2585_Y167F

   Plasmid

   #174398

   Purpose

   Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   Y167F

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Flag-gp130dimer-stm2585

   Plasmid

   #174395

   Purpose

   Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   IL6ST cytosolic domain and sarA chimera
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-Flag-stm2585-gp130

    Plasmid

    #174393

    Purpose

    Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA-IL6ST chimera
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    140-177 of sarA replaced with homologous IL6ST se…

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Flag-stm2585_Y167F

    Plasmid

    #174392

    Purpose

    S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    stm2585
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Tyr 167 changed to Phe

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Flag-stm2585

    Plasmid

    #174391

    Purpose

    S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    stm2585
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-R2L3-IRES-bgeo-pA

    Plasmid

    #139533

    Purpose

    Provides IRES-bgeo-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    bgeo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-R2L3-cmER-IRESpuro-pA

    Plasmid

    #139539

    Purpose

    Provides mouse ER protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-CreStopDeleted

    Plasmid

    #139546

    Purpose

    Cre ORF without stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-mKO1

    Plasmid

    #139553

    Purpose

    mKO1 ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR-L5L2-mCherry-stop

    Plasmid

    #139560

    Purpose

    mCherry ORF with stop codon between attL5-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-L4R1-hCMVstar1+bgloInt

    Plasmid

    #139528

    Purpose

    Provides conditional promoter cassette between attL4-attR1 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    hCMVstar1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-R2L3-cHA-IRESpuro-pA

    Plasmid

    #139537

    Purpose

    Provides HA protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-d4Venus

    Plasmid

    #139552

    Purpose

    d4Venus ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    d4Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge