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Addgene
Showing: 41 - 60 of 7840 results

  10. mApple-proα1(I)

    Plasmid
    #119844
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    119844-plasmid-map-sequence-id-233910

    Enlarge

  11. mApple-proα2(I)-L

    Plasmid
    #120161
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    120161-plasmid-map-sequence-id-233911

    Enlarge

  12. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Erik Rodriguez, Roger Tsien
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  14. p3E-FAPP1

    Plasmid
    #109555
    Purpose
    Multisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    109555-plasmid-map-sequence-id-222033

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  15. mT-Sapphire-CytERM-N-17

    Plasmid
    #56278
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 511
    Depositor
    Michael Davidson
    Insert
    CytERM
    Use
    Tags
    mT
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    56278-plasmid-map-sequence-id-183147

    Enlarge

  16. mT-Sapphire-H2B-6

    Plasmid
    #56404
    Purpose
    Localization: Nucleus/Histones, Excitation: 399, Emission: 511
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    mT
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mApple-Display-5

    Plasmid
    #54543
    Purpose
    Localization: Cytoplasm, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mApple-Display
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  20. mApple-Peroxisomes-2

    Plasmid
    #54627
    Purpose
    Localization: Peroxisomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Peroximal Targeting Signal 1 (PTS1) and mApple
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 60 of 7840 results