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Plasmid#8393DepositorInsertdeletion
UseCre/LoxTagsExpressionMammalianMutation1 kb EcoRI to HindIII deletion of pBS185.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Luc-N
Plasmid#58790PurposeExpress LucN alone in mammalian cellsDepositorInsertfirefly Luciferase 1-398
UseTagsFLAGExpressionMammalianMutationT576C,T591C to destroy MfeI and EcoRI sitesPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-Ef1a-EcoR1
Plasmid#203311PurposeFor expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pG5ElbLuc
Plasmid#89341PurposeThe plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQFT-lacZ
Plasmid#208896PurposepQFT with lacZ under control of PQJDepositorInsertlacZ
UseTagsExpressionBacterialMutationContains two silent mutations and a missense muta…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-miR9 Sensor
Plasmid#22742DepositorInsertmicroRNA 9 binding sites
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-CMV-EcoR1
Plasmid#203305PurposeFor expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rp51a Hyperstabilized 5'SS
Plasmid#100988PurposepUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGTDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut ATG/GTATGT -> AAG/GTAAGTPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-67Sma
Plasmid#49342PurposeChlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic regionDepositorInsertEcoRI 5.8 kB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIM1708-surT-PsurP-agaN-pNW33N
Plasmid#44009DepositorInsertsurT-PsurP-agaN
UseTagsExpressionBacterialMutationDeleted EcoRI site (synonymous change, C to T at …PromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
MXS_MCS2
Plasmid#62396PurposeMXS_chaining vector with MCS2DepositorInsertEcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HMEKc-srcM
Plasmid#1107DepositorInsertc-src
UseTagsExpressionYeastMutationMET3 promoter inserted in EcoRI and BamHI of pRS3…PromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-NoBS-NoPseudoBS
Plasmid#100986PurposeRP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationBS mutated to GTTAGTG, pseudo BS mutated to GTTTG…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-hrDNA-12.0
Plasmid#206320PurposeA plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)DepositorInsertIntergenic spacer of human rDNA repeat
UseCloningTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJM614-TRA-2::GFP
Plasmid#27128DepositorInsertTRA-2 GFP (tra-2 Nematode)
UseTagsGFPExpressionWormMutationreplaced stop codon with AgeI and EcoRI added GFP…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
UseTagsExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only