We narrowed to 226 results for: lbcas12a

1. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

   Plasmid

   #160629

   Purpose

   TU for the expression of LbCas12a

   Depositor

   Diego Orzaez

   Insert

   AtUBQ10-:LbCpf1(Cas12a):Tnos
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   UBQ10

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

   Plasmid

   #244838

   Purpose

   Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Flex-Cas12a
   

   Use

   CRISPR

   Tags

   2xSV40 NLS

   Expression

   Mammalian

   Mutation

   G146R/R182V/D535G/S551F/D665N/E795Q

   Promoter

   T7

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

   Plasmid

   #244204

   Purpose

   Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

   Plasmid

   #244210

   Purpose

   Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SM268_EF1A-dHyperLbCas12a-P300-T2A-EGFP

   Plasmid

   #244206

   Purpose

   Mammalian expression of dHyperLbCas12a-P300 with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-P300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SM269_EF1A-dHyperLbCas12a-P300-T2A-BlastR

   Plasmid

   #244207

   Purpose

   Mammalian expression of dHyperLbCas12a-P300 with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-P300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. SM266_EF1A-dHyperLbCas12a-KRAB-T2A-BlastR

   Plasmid

   #244205

   Purpose

   Mammalian expression of dHyperLbCas12a-KRAB with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFP

   Plasmid

   #244208

   Purpose

   Mammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-4xSID
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. SM273_EF1A-dHyperLbCas12a-VPR-T2A-BlastR

   Plasmid

   #244211

   Purpose

   Mammalian expression of dHyperLbCas12a-VPR with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA

    Plasmid

    #117410

    Purpose

    LbCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    LbCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid

    #114070

    Purpose

    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid

    #140197

    Purpose

    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dLbCas12a-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNase deactivated

    Promoter

    PgpdA

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid

    #140193

    Purpose

    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    

    Use

    CRISPR and Synthetic Biology; Fungal genome integ…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNAse deactivated

    Promoter

    PgpdA and PtrpC

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag

    Plasmid

    #236386

    Purpose

    Overexpression of HyperdLbCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D156R, D235R, E292R, D350R

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

    Plasmid

    #236368

    Purpose

    Overexpression of HyperdLbCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D156R, D235R, E292R, D350R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid

    #114085

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid

    #114086

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CHyMErA LbCas12a Nuclease Optimization hgRNA Library

    Pooled Library

    #209734

    Purpose

    CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different LbCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

20. pYPQ230-RRV

    Plasmid

    #188543

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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