We narrowed to 9,226 results for: sacs

1. pMA7-SacB

   Plasmid

   #79968

   Purpose

   TM-MAGE strain

   Depositor

   Morten Sommer

   Article

   Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

   Inserts

   Lambda Red recombinase beta subunit
   DNA adenine methylase
   Levansucrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BA31_pU.CAG.SaCas9-D10A.rBGpA

   Plasmid

   #199251

   Purpose

   Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SaCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-sACE2(WT)

   Plasmid

   #145147

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SQ171fg/pCSacB

   Plasmid

   #69344

   Purpose

   SQ171 fast growing strain supported by pCSacB plasmid

   Depositor

   Michael Jewett

   Article

   Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

   Insert

   pCSacB
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. SaCas9 TadDE

   Plasmid

   #193846

   Purpose

   Express TadDE (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE with SaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SaCas9v3-Puro

   Plasmid

   #178813

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET22b-CPDSacI

   Plasmid

   #38255

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-mCherry-C1-PsACR1

   Plasmid

   #85465

   Purpose

   Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

   Insert

   Synthetic construct PsChR1 gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+enhancer)

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. SaCas9 TadCBEa

    Plasmid

    #193844

    Purpose

    Express TadCBEa (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    SaCas9 TadCBEa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. dSaCas9-KRAB

    Plasmid

    #205250

    Purpose

    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

    Depositor

    Rasmus Bak

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

    Plasmid

    #116963

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQF-mNGsacB

    Plasmid

    #203673

    Purpose

    pQF with mNeonGreen and sacB under control of PQ5

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Has synonymous (silent) mutations to remove BsrGI…

    Promoter

    PQ5

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CSAC-Pers

    Plasmid

    #168281

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CSAC-Crys

    Plasmid

    #164967

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    hU6, hSyn

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

    Plasmid

    #116965

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ABEMax nSaCas9

    Plasmid

    #135364

    Purpose

    Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax nSaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

    Plasmid

    #116964

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CSAC-Bmal1

    Plasmid

    #168296

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgBmal1-1-hU6-sgBmal1-3
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sACE2v2

    Plasmid

    #145149

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge