We narrowed to 11,298 results for: sacs

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pMC-9-OsAct2-11

Plasmid

#197765

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsAct2 terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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SaCas9v3-Puro

Plasmid

#178813

Purpose

SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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dSaCas9-KRAB

Plasmid

#205250

Purpose

In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

Depositor

Rasmus Bak

Insert

dSaCas9-KRAB

Use

CRISPR

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadCBEd

Plasmid

#193845

Purpose

Express TadCBEd (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SaCas9

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-SaCas9

Plasmid

#129553

Purpose

Contains codon-optimized SaCas9 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

SaCas9

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-RsACE2

Plasmid

#159665

Purpose

Expresses Rhinolophus sinicus ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Level 1 P1 OsActinP:Hygint:NosT

Plasmid

#165423

Purpose

GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator

Use

Synthetic Biology; L1 p1(r) golden gate (moclo) h…

Promoter

Os Actin promoter :: Hygromycin resistance gene (…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)

Plasmid

#145147

Purpose

Mammalian expression plasmid for soluble ACE2 (protease domain)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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SaCas9v2-Puro

Plasmid

#178802

Purpose

SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SQ171fg/pCSacB

Plasmid

#69344

Purpose

SQ171 fast growing strain supported by pCSacB plasmid

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

pCSacB

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadDE

Plasmid

#193846

Purpose

Express TadDE (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE with SaCas9

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDSacI

Plasmid

#38255

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-PsACR1

Plasmid

#85465

Purpose

Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

Insert

Synthetic construct PsChR1 gene

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadCBEa

Plasmid

#193844

Purpose

Express TadCBEa (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

SaCas9 TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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CSAC-Pers

Plasmid

#168281

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

Plasmid

#116965

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pQF-mNGsacB

Plasmid

#203673

Purpose

pQF with mNeonGreen and sacB under control of PQ5

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQ5

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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