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pRepair-SGFP2-CTNNB1

Plasmid

#153430

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SGFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-CTNNB1-ATG

Plasmid

#153429

Purpose

Expresses a gRNA that overlaps the startcodon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CTNNB1:1-781

Plasmid

#211024

Purpose

Mammalian expression of full-length human CTNNB1. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CTNNB1:M1-L781 (CTNNB1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-CTNNB1-S45

Plasmid

#164587

Purpose

Expresses a gRNA that overlaps the S45 codon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRepair-mScI-CTNNB1

Plasmid

#153431

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mScarlet-i

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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J8AAV-HDR Ctnnb1.1

Plasmid

#73452

Purpose

J8AAV-HDR U6sgCtnnb1.1 template

Depositor

Wen Xue

Article

Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

AAV

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-HA-Luciferase

Plasmid

#227803

Purpose

cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of luciferase and a HA tag

Depositor

Yuxuan Guo

Article

Chen et al Fundam Res. 2025 Jun 30;5(6):2993-3000.

Insert

HA-Luciferase

Use

AAV

Expression

Mammalian

Promoter

Tnnt2

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_E307K

Plasmid

#234560

Purpose

CTNNA1 butterfly eye mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_E307K (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

E307K

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_L436P

Plasmid

#234559

Purpose

CTNNA1 eye phenotype via forward genetic screen

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_L436P (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

L436P

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_R551A

Plasmid

#234558

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_R551A (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

R551A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-TNNI2-Brd 37

Plasmid

#219019

Purpose

Transcription factor TNNI2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

TNNI2 (TNNI2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - G752A in pENTR

Plasmid

#216623

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Mutation

G752A

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R673w in pENTR

Plasmid

#216622

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Mutation

R673W

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - T551M in pENTR

Plasmid

#216621

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Mutation

T551M

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R565C in pENTR

Plasmid

#216620

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Mutation

R565C

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R376H in pENTR

Plasmid

#216619

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Mutation

R376H

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - W388S in pENTR

Plasmid

#216618

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Mutation

W388S

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.2-GFP

Plasmid

#209098

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.2

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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