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Plasmid#113741PurposeGateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1R5
Plasmid#113737PurposeGateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1L2
Plasmid#113735PurposeGateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-C
Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Xl-VSP1-GFP
Plasmid#51883Purposeexpression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 (tpte2 Frog)
UseXenopus oocyte expressionTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-GFP-Msp300KASH
Plasmid#170806PurposeExpresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesisDepositorInsertGFP-Msp300KASH
UseTagsGFPExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xl-VSP1 C301S
Plasmid#51882Purposeexpression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 C301S (tpte2 Frog)
UseXenopus oocyte expressionTagsExpressionMammalianMutationC301S, catalytically-inactive mutant of Xl-VSP1PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mEGFP-N
Plasmid#113746PurposeGateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP
UseCRISPR; Gateway entry vectorTagsmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mEGFP-C
Plasmid#113749PurposeGateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP
UseCRISPR; Gateway entry vectorTagsmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-C
Plasmid#113755PurposeGateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-C
Plasmid#113757PurposeGateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-N
Plasmid#113752PurposeGateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-N
Plasmid#113747PurposeGateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-N
Plasmid#113748PurposeGateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-C
Plasmid#113750PurposeGateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-N
Plasmid#113753PurposeGateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_18GZG
Plasmid#117228Purposeshble flanked by an endogenous GAPDH promoter and terminator systemDepositorInsertshble
UseThraustochytrid expressionTagsExpressionMutationPromoterGAPDHAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-neo
Plasmid#155026PurposeDinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) geneDepositorInsertamino 3'-glycosyl phosphotransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only