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myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.23 A82V 2014 EBOV + Mucin-Like Domain

Plasmid

#86016

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86021

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain

Promoter

CMV IE

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK14-P2A-Hygro_Barcode

Plasmid

#170239

Purpose

Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK14 (MAPK14 Human)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK1-P2A-Hygro_Barcode

Plasmid

#170221

Purpose

Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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1D3 HC

Plasmid

#170670

Purpose

Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)

Depositor

Ronald Jemmerson, Rafel Radi

Article

Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

Insert

HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody

Expression

Mammalian

Available Since

July 12, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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1D3 LC

Plasmid

#170669

Purpose

Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)

Depositor

Ronald Jemmerson, Rafel Radi

Article

Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

Insert

LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody

Expression

Mammalian

Available Since

June 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-HtrA2-FLAG S400D

Plasmid

#15940

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S400D

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

Plasmid

#170217

Purpose

Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CASP8 (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-delta 86-105

Plasmid

#78922

Purpose

Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 86-105

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only