We narrowed to 1,403 results for: PTS;

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MiCy-C1

Plasmid

#54609

Purpose

Localization: C1 Cloning Vector, Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

MiCy

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pGL3b(1454/3172)P2P-mut

Plasmid

#21405

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

hypoxia response element mutated

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
cmamxA1-GFP

Plasmid

#107195

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A1

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-YFP-ICP0FxE

Plasmid

#134564

Purpose

Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

YFP-ICP0

Tags

None

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArC K72R

Plasmid

#59811

Purpose

Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Tags

Myc

Expression

Mammalian

Mutation

K72R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pXCX.Syn.EGFP

Plasmid

#38054

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

Synapsin (human SYN1)

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pNIC28-BpWcbI

Plasmid

#125591

Purpose

Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

Insert

BpWcbI (wcbI Burkholderia pseudomallei)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm2

Plasmid

#99356

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm2

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM2 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP1FBAR(516a.a.)-EGFP

Plasmid

#168505

Purpose

Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP1-FBAR (SRGAP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-516

Promoter

CAG

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-KCC2

Plasmid

#104075

Purpose

expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

Depositor

Igor Medyna

Article

Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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GST-RNF126 C231A

Plasmid

#138644

Purpose

Bacterial expression of GST-RNF126 RING mutant (C231A) fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C231A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pmKikGR Actin

Plasmid

#105315

Purpose

Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

Depositor

Kenneth Yamada

Article

Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

Insert

actin beta (ACTB Human)

Tags

KikGR

Expression

Mammalian

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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exon K-renilla

Plasmid

#81007

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Tags

Renilla luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSEW-Venus-Sec16A-deltaN

Plasmid

#66605

Purpose

Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec16A (SEC16A Human)

Tags

Venus (EYFP derivative)

Expression

Mammalian

Mutation

encodes amino acids 1107-1217 of human Sec16A

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a D540K

Plasmid

#53057

Purpose

Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Aspartate 540 to Lysine

Promoter

SP6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pJES103

Plasmid

#135410

Purpose

E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter

Depositor

Miriam Braunstein

Article

McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

Insert

blaTEM-1

Expression

Bacterial

Mutation

E. coli 'blaTEM-1 with M. tuberculosis mpt63…

Promoter

hsp60

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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KBB460

Plasmid

#185094

Purpose

NMA111-GFP wild type under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP3FBAR-EGFP

Plasmid

#168506

Purpose

Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP3-FBAR (SRGAP3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-492

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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