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Showing: 581 - 600 of 1425 results

  2. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  3. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Mutation
    Promoter
    pdx
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

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  4. enIscB

    Plasmid
    #205410
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    205410-plasmid-map-sequence-id-421947

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  5. enIscB-T5E

    Plasmid
    #205411
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205411-plasmid-map-sequence-id-416557

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  8. pRegev_ExonSkip_Backbone

    Plasmid
    #204187
    Purpose
    Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    First exon and intron for beta globin splicing minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    204187-plasmid-map-sequence-id-404040

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  9. PB-FLuc+GFPd2

    Plasmid
    #127190
    Purpose
    Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)
    Depositor
    Jordan Green
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127190-plasmid-map-sequence-id-248023

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  10. TRE-MCS-WPRE-SV40 - GCH25

    Plasmid
    #230826
    Purpose
    AAV cloning vector for expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230826-plasmid-map-sequence-id-459123

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  11. Syn-MCS-WPRE-SV40 - GCH7

    Plasmid
    #230817
    Purpose
    AAV cloning vector for expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230817-plasmid-map-sequence-id-457138

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  12. CaMKII-MCS-WPRE-SV40 - GCH19

    Plasmid
    #230823
    Purpose
    AAV cloning vector for expression under the CaMKII promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230823-plasmid-map-sequence-id-457140

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  13. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid
    #230820
    Purpose
    AAV cloning vector for expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230820-plasmid-map-sequence-id-457137

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  14. GFAP-MCS-WPRE-SV40 - GCH31

    Plasmid
    #230829
    Purpose
    AAV cloning vector for expression under the GFAP promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230829-plasmid-map-sequence-id-457134

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  15. pPBC-G6s

    Plasmid
    #62812
    Purpose
    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62812-plasmid-map-sequence-id-123114

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  16. pPBC-LG6s

    Plasmid
    #62809
    Purpose
    pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62809-plasmid-map-sequence-id-123119

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  17. pPBC-G6f

    Plasmid
    #62811
    Purpose
    pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62811-plasmid-map-sequence-id-123116

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  18. pPBC-LG6f

    Plasmid
    #62808
    Purpose
    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    62808-plasmid-map-sequence-id-123121

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  19. pPBC-G3-tdT

    Plasmid
    #62814
    Purpose
    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62814-plasmid-map-sequence-id-121226

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  20. Syn-FRT-MCS-WPRE-SV40 - GCH9

    Plasmid
    #230819
    Purpose
    AAV cloning vector for Flpo dependent expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230819-plasmid-map-sequence-id-459150

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Showing: 581 - 600 of 1425 results