Addgene

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pVP184

Plasmid

#222051

Purpose

GreenGate EF bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP185

Plasmid

#222052

Purpose

GreenGate BF bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP186

Plasmid

#222053

Purpose

GreenGate CF bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP224

Plasmid

#222054

Purpose

GreenGate AC bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP225

Plasmid

#222055

Purpose

GreenGate AD bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP084

Plasmid

#222036

Purpose

meffBlue chromoprotein in a MultiGreen 1.0 HG entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

meffBlue

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP085

Plasmid

#222037

Purpose

spisPink chromoprotein in a MultiGreen 1.0 HG entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

spisPink

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP086

Plasmid

#222038

Purpose

mRFP1e chromoprotein in a MultiGreen 1.0 HG entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pVP088

Plasmid

#222039

Purpose

MultiGreen 1.0 HG entry vector containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP089

Plasmid

#222040

Purpose

MultiGreen 1.0 base HG entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

H and G overhangs for GreenGate assembly

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP090

Plasmid

#222041

Purpose

MultiGreen 1.0 FH entry vector containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP091

Plasmid

#222042

Purpose

MultiGreen 1.0 base FH entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Tags

Expression

Mutation

F and H overhangs for GreenGate assembly

Promoter

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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P30.pA.NGFR-mSrtA.mCMV.hPGK.GFP.wpre

Plasmid

#226728

Purpose

backbone vector, GFP expression

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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insulator_seq_barcode_vector

Plasmid

#221458

Purpose

Vector for generating a barcode library for insulator-seq.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Type

Empty backbone

Use

Synthetic Biology and Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRMCD77

Plasmid

#173032

Purpose

Modified pET17b (Novagen) vector

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207-MpPHOT

Plasmid

#100600

Purpose

Gateway entry vector for MpPHOTOTROPIN

Depositor

Yutaka Kodama

Article

Kodama et al PLoS One. 2016 Mar 30;11(3):e0152484. do

Insert

MpPHOT

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

Plasmid

#202762

Purpose

Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2 (NEUROG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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P527_POU4F2-p2A-mNeonGreen-h2b

Plasmid

#239103

Purpose

Azenta NGS; inserts a p2A-mNeonGreen-h2b fusion at the endogenous POU4F2 (aka BRN3B) gene

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

POU4F2 (POU4F2 Human)

Use

Tags

p2A-mNeonGreen-h2b

Expression

Mammalian

Mutation

Promoter

Endogenous POU4F2

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123A)-EYFP (AAV9)

Viral Prep

#35505-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(E123A)-EYFP (#35505). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(E123A)-EYFP plasmid DNA. CaMKIIa-driven hChR2(E123A)-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Promoter

CaMKII

Tags

EYFP

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only
TP53 MITE Library

Pooled Library

#113569

Purpose

Pooled lentiviral plasmid library containing ~8,000 single amino acid substitution and nonsense mutant variants of p53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only