Addgene

We narrowed to 4,305 results for: TIL;

Showing: 581 - 600 of 4305 results

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pSL2680

Plasmid

#85581

Purpose

Replicating base vector for constructing cpf1/CRISPR editing plasmids

Depositor

Himadri Pakrasi

Article

Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEPkan-S2

Plasmid

#61601

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pV1393:SpRY

Plasmid

#172189

Purpose

Genome editing of C. albicans

Depositor

Doug Bernstein

Article

Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p5-39

Plasmid

#118088

Purpose

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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P48

Plasmid

#212155

Purpose

Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Insert

superfolder green fluorescent protein, secondary alcohol dehydrogenase

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

PgyrA

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pIB2

Plasmid

#25451

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET-NDH6

Plasmid

#105503

Purpose

Expression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

NDH6

Use

Tags

6xHis

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCM66

Plasmid

#45827

Depositor

Mary Lidstrom

Article

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Broad host range cloning vector in bacteria

Tags

Expression

Mutation

Promoter

lac

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pIB4

Plasmid

#25453

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pBP-ORF-xylE

Plasmid

#112780

Purpose

Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

xylE open reading frame

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB

Plasmid

#11777

Depositor

Didier Trono, Patrick Aebischer

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1

Plasmid

#41023

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1

Use

Promoter replacement

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Use

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pIVT

Plasmid

#122139

Purpose

For making cRNA appropriate for microinjection

Depositor

Carmen Williams

Article

Igarashi et al Dev Biol. 2007 Dec 1;312(1):321-30. Epub

Type

Empty backbone

Use

For in vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMK-Prrn (N. tabacum)

Plasmid

#123490

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

Prrn (N. tabacum)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFIP

Plasmid

#17565

Depositor

Jay Keasling

Article

Ham et al Biotechnol Bioeng. 2006 May 5. 94(1):1-4

Insert

inversion switch

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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Tg(lyz:EGFP-Mmu.Rpl10a)

Plasmid

#128838

Purpose

RNA isolation from neutrophils via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-GGAC-ATGC

Plasmid

#60949

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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PRM-GFP

Plasmid

#40127

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

CI inducible sfGFP

Use

Synthetic Biology

Tags

LVA ssrA tag

Expression

Bacterial

Mutation

Promoter

pRM

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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