We narrowed to 7,980 results for: ara-2

1. pfMDH_R373A

   Plasmid

   #67731

   Purpose

   Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed arginine 373 to alanine

   Promoter

   T7

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pSBL_DK186

   Plasmid

   #156422

   Purpose

   Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein
   

   Use

   Tags

   EGFP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSBL_DK198

   Plasmid

   #156425

   Purpose

   Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (aa 42-275)
   

   Use

   Tags

   sfGFP-His

   Expression

   Insect

   Mutation

   aa 42-275

   Promoter

   Polyhedrin

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGADT7-GW

   Plasmid

   #61702

   Purpose

   Gateway compatible Y2H vector - AD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBKT7-GW

   Plasmid

   #61703

   Purpose

   Gateway compatible Y2H vector - BD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-Bmpr2ΔKinase

   Plasmid

   #163404

   Purpose

   Expresses BMPR-2 lacking the kinase domain in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pfMDH_WT

   Plasmid

   #67721

   Purpose

   Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMCSG53_Nsp1

   Plasmid

   #167256

   Purpose

   Bacterial expression of Nsp1 residues 13-127

   Depositor

   Alexei Savchenko

   Article

   Semper et al iScience. 2021 Jan 22;24(1):101903. doi:

   Insert

   Nsp1 (residues 13-127) (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-TEV site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   April 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCAG-Bmpr2Δ751-813aa

   Plasmid

   #163603

   Purpose

   Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The LIMK-binding region (751-813 aa) is deleted

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pSBL_DK187

    Plasmid

    #156423

    Purpose

    Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBL_DK193

    Plasmid

    #156424

    Purpose

    Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (Q57H)
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Q57H

    Promoter

    Polyhedrin

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pfMDH_Triple

    Plasmid

    #67733

    Purpose

    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed histidine 303 to alanine, arginine 373 to…

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSBL_DK202

    Plasmid

    #156426

    Purpose

    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Use

    Tags

    EGFP-His

    Expression

    Mammalian

    Mutation

    aa 42-275

    Promoter

    CMV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ5080_pHR_PGK_sfGFP_CoV-F2

    Plasmid

    #155304

    Purpose

    SARS-CoV-2 fluorescent reporter 2

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Superfolder GFP fused with SARS-CoV-2 fluorescent reporter 2 (N, ORF1ab Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-iLOV-Rsn2

    Plasmid

    #61687

    Purpose

    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV

    Depositor

    Brian Smith

    Insert

    Ranaspumin-2
    

    Use

    Tags

    His and iLOV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-tetO-Tfap2a

    Plasmid

    #70274

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Tfap2a (Tfap2a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGTQL1211YN

    Plasmid

    #61704

    Purpose

    Gateway compatible BiFC vector (pEarleyGate201-YN)

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Bifc assay

    Tags

    YFP-N and HA

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGTQL1221YC

    Plasmid

    #61705

    Purpose

    Gateway compatible BiFC vector (pEarleyGate202-YC)

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Bifc assay

    Tags

    YFP-C and FLAG

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid

    #159364

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge