Addgene

We narrowed to 2,752 results for: fas

Showing: 581 - 600 of 2752 results

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PX543 SpCas9(del175-307)

Plasmid

#58888

Purpose

Truncated form of SpCas9 without REC2 domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 175-307

Promoter

Available since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE1-VP64-P10

Plasmid

#104606

Purpose

Expresses TALE1 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE1

Use

TALEN

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE4-VP64-P10

Plasmid

#104608

Purpose

Expresses TALE4 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE4

Use

TALEN

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K403A

Plasmid

#195612

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

A1463G, A1464C

Promoter

T7

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22YU3-KO

Plasmid

#170313

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22YU3 knockout

Use

Tetrahymena somatic gene knockout vector

Tags

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22MS1-KO

Plasmid

#170312

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22MS1 knockout

Use

Tetrahymena somatic gene knockout vector

Tags

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pACEBac1-Q22MS1-2xStrep

Plasmid

#170314

Purpose

Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22MS1

Use

Tags

Strep-Tag II

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP (PX458)

Plasmid

#48138

Purpose

Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-EGFP-Rac1-Q61L

Plasmid

#12981

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 constitutively active (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO.G76V

Plasmid

#11932

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin KO.G76V (RBX1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Conjugation deficient mutant. All 7 lysines of ub…

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pBluescript-IC3-FLAG-Tev-ZZ

Plasmid

#170311

Purpose

Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Outer Dynein Arm Intermediate Chain 3

Use

Tags

FLAG, ZZ

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-T17N

Plasmid

#12982

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 DN (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAIP

Plasmid

#74171

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS3-Syn21-V5-mCherry-P10

Plasmid

#104617

Purpose

Expresses V5 tagged mCherry upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

Syn21-V5-mCherry-P10-MhcSA

Use

Tags

V5

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS

Plasmid

#90996

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSO221 Phis-72 BirA GFP

Plasmid

#45100

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

BirA::GFP
unc-119 (unc-119 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

his-72 and unc-119

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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