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hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pCD/NL-BH∆1

Plasmid

#41791

Purpose

Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

HIV-1 Gag/Pol and RRE sequences

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP670nano

Plasmid

#127441

Purpose

G protein alpha-s internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

G protein alpha-s-miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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FnR9-10

Plasmid

#182567

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KG#62

Plasmid

#110874

Purpose

Expresses the C. elegans acy-1 cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

Inserts

rab-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-2mCh-PS

Plasmid

#112268

Purpose

IDOCKS Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

two copies of mCherry followed by a pseudosubstrate peptide for PKC

Tags

mCh

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Fluc/CMV/WPREdU3

Plasmid

#136881

Purpose

Reporter vector expression firefly luciferase

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Firefly luciferase

Use

Lentiviral

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)EGFP/CMV/WPRE∆U3

Plasmid

#41790

Purpose

Lentiviral vector for CMV promoter driven expression of EGFP

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

EGFP

Use

Lentiviral

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
TfRΔEctoR1-eGFP A206K

Plasmid

#113554

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-eGFP A206K

Expression

Mammalian

Mutation

eGFP A206K

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pGFP Glra2 (CC#301)

Plasmid

#15199

Depositor

Connie Cepko

Article

Young et al Neuron. 2004 Mar 25. 41(6):867-79.

Insert

Glra2 (GLRA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR-29b-1~29a

Plasmid

#51376

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

pri-miR-29b-1~29a (MIR29B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pBB14

Plasmid

#18657

Depositor

Lynn Enquist

Article

Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

Insert

Us9-EGFP gene fusion

Tags

EGFP

Expression

Mammalian

Mutation

First 106 amino acids of Us9 are intact. The stop…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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p1.2-Hygro

Plasmid

#162741

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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p1.2-Neo

Plasmid

#162739

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E15: attB4-EF1p>-attB1r

Plasmid

#162920

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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