We narrowed to 19,554 results for: iNOS

1. GFP-RAPH1Δ536-587

   Plasmid

   #194865

   Purpose

   Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   RAPH1 with amino acids 536-587 deleted

   Promoter

   CMV

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-M.Fas.2

   Plasmid

   #196688

   Purpose

   Rep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.

   Depositor

   Pardis Sabeti

   Article

   Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   STIPTMK insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174881

   Purpose

   Targeting vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Flnc
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174885

   Purpose

   Targeting vector for generating Usp5 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Usp5
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174886

   Purpose

   Targeting vector for generating Wnk1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Wnk1
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Chinchilla–insM

   Plasmid

   #191226

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   removed methionine at 122 in the protein sequence

   Promoter

   PH and p10

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Chinchilla+N122D–insM

   Plasmid

   #191227

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   removed methionine at 122 and replaced asparagine…

   Promoter

   PH and p10

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sandgrouse–insD

   Plasmid

   #191228

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   removed aspartic acid at 121 in the protein seque…

   Promoter

   PH and p10

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-v2-ALDH1A3gRNA2

   Plasmid

   #189747

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-v2-ALDH1A3gRNA1

    Plasmid

    #189746

    Purpose

    The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    Cas9, ALDH1A3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CMV-Cyp26a1

    Plasmid

    #185563

    Purpose

    AAV construct to ubiquitously express mouse Cyp26a1

    Depositor

    Connie Cepko

    Article

    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

    Insert

    Cyp26a1 (Cyp26a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FluoSTEP-AKAR (C/Y)

    Plasmid

    #181862

    Purpose

    FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AKAR (C-Y)
    

    Use

    Tags

    CFP(1-10) and cpVenus(E127)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Prom1 Del AA 6

    Plasmid

    #184024

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 689 to 694.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8 Del AA 6 (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 689 to 694

    Promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Prom1 D-1

    Plasmid

    #184013

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 685-706.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-1 (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 685-706.

    Promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid

    #182305

    Purpose

    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    

    Use

    Tags

    A modified TorA periplasmic targeting sequence pl…

    Expression

    Bacterial

    Mutation

    Spontaneous mutation of calmodulin's penulti…

    Promoter

    t7

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TMD18

    Plasmid

    #163673

    Purpose

    Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Promoter

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMD19

    Plasmid

    #163674

    Purpose

    Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Promoter

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FKBP-ST*-GFP

    Plasmid

    #163669

    Purpose

    Expresses FKBP tagged partial length ST in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    

    Use

    Tags

    FK506 binding protein (FKBP) and GFP

    Expression

    Mammalian

    Mutation

    This chimera contains the first 113 amino acids o…

    Promoter

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLIC-His-GBAi W211A

    Plasmid

    #171756

    Purpose

    For the expression of His-GBAi W211A in bacteria

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. OA-1010A

    Plasmid

    #176078

    Purpose

    pBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRed

    Depositor

    Omar Akbari

    Article

    Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

    Insert

    L596_g9608.t1
    

    Use

    Tags

    3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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