We narrowed to 13,268 results for: ALP

1. Human b4 nicotinic receptor subunit EM construct

   Plasmid

   #163961

   Purpose

   pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

   Depositor

   Ryan Hibbs

   Article

   Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

   Insert

   Human beta4 nicotinic acetylcholine receptor subunit EM construct
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   StrepII tag and apocytochrome b(562)RIL (BRIL)

   Expression

   Mammalian

   Mutation

   Pro341-Ser395 replaced with apocytochrome b(562)R…

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0499

   Plasmid

   #143881

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARGC1A (PPARGC1A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ECE1-His-bio

   Plasmid

   #53361

   Purpose

   Expresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ECE1 (ECE1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-HSPA5P

   Plasmid

   #44150

   Purpose

   a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

   Depositor

   Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   HSPA5 promoter (HSPA5 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLV-shEsr2

   Plasmid

   #120722

   Purpose

   Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

   Depositor

   Amy Lasek

   Article

   Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

   Insert

   Esr2 (Esr2 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC/CMV-TYK2K930R-VSV

   Plasmid

   #139346

   Purpose

   Expression of kinase-dead TYK2-K930R protein

   Depositor

   Sandra Pellegrini

   Article

   Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-K930R

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

   Plasmid

   #33255

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and phenylalan…

   Promoter

   SV40

   Available Since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCR3-FLAG-Gephyrin GC

   Plasmid

   #68818

   Purpose

   Flag tagged mammalian expression of GC domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NIL

   Promoter

   CMV

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. F2R-bio-His

   Plasmid

   #51863

   Purpose

   Expresses full-length Proteinase-activated receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F2R (F2R Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-FKBP-TPD54(R159E)

    Plasmid

    #172453

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutant

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    changed Arginine 159 to Glutamic Acid

    Promoter

    CMV

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. VP16-ER beta (short)

    Plasmid

    #11353

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER beta short (ESR2 Human)
    

    Expression

    Mammalian

    Available Since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA6-N-3XFLAG-Fzd6

    Plasmid

    #123588

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    frizzled class receptor 6 (Fzd6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. FB009

    Plasmid

    #119707

    Purpose

    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC::nptII::Ttub
    

    Use

    Synthetic Biology

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TTMP-His-bio

    Plasmid

    #52335

    Purpose

    Expresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TTMP (C3orf52 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. INSR-bio-His

    Plasmid

    #53341

    Purpose

    Expresses full-length Insulin receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    INSR (INSR Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUHD-HA-AML1-ETO

    Plasmid

    #12430

    Depositor

    Dong-Er Zhang

    Article

    Rhoades et al Blood. 2000 Sep 15. 96(6):2108-15.

    Insert

    AML1-ETO (RUNX1, RUNX1T1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    BstXI-SacII fragment in pUHD-AML1-ETO replaced wi…

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pPKm-230

    Plasmid

    #90499

    Purpose

    pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tFD and mito-tFNR
    

    Use

    Lentiviral

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX neo ACTN1-GFP, Y246E

    Plasmid

    #138294

    Purpose

    Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    ACTN1 (ACTN1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y246E

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTER GAMMA-TUBULIN shRNA Human

    Plasmid

    #87955

    Purpose

    reduced the expression of gamma-tubulin in human cells

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    sh gamma-tubulin (TUBG1 Human)
    

    Tags

    No-tag

    Expression

    Mammalian

    Mutation

    The protein is a sh-gamma-tubulin resistant gene.…

    Available Since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJO427

    Plasmid

    #111139

    Purpose

    URT-type vector for Glu-mIκBα expression in Mammalian cells

    Depositor

    Alexander Varshavsky

    Article

    Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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