We narrowed to 20,184 results for: LEA

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II DC

Plasmid

#35181

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal domain repeats 1-22 and 31-52

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pKDC.015

Plasmid

#184992

Purpose

Express -Eco2 RT and ncRNA in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(wt), a1/a2 length: 13

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGP4G-TPLRD1-ccdB

Plasmid

#83877

Purpose

Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670

Plasmid

#82621

Purpose

Expresses NLS-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670

Use

Piggybac vector

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-FKBP-mTagBFP2-MCS

Plasmid

#178864

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF102

Plasmid

#13236

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF102

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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IZ1181

Plasmid

#21049

Depositor

Tadahide Izumi

Article

Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.

Insert

human MDM2 (MDM2 Human)

Tags

no tag

Expression

Mammalian

Mutation

no mutation. This is the wild-type human MDM2.

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only
pTAL3

Plasmid

#31034

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALEN-FokI nuclease backbone

Tags

AcV5 and FokI nuclease

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–EgRNA

Plasmid

#62716

Purpose

Empty; no U6 gRNA cassette

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pHJ346

Plasmid

#20678

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pIS0 - HOXA7 3'UTR

Plasmid

#12079

Depositor

David Bartel

Article

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Insert

HOXA7 3'UTR (HOXA7 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ccdB

Plasmid

#188445

Purpose

Cloning Vector and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIS0 - HOXB8 3'UTR

Plasmid

#12076

Depositor

David Bartel

Article

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Insert

HOXB8 3'UTR (HOXB8 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pTC208

Plasmid

#70017

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Expression

Plant

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTORKm43GW

Plasmid

#112897

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418).

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-MseLig1

Plasmid

#107071

Purpose

Expresses M. sedula DNA Ligase 1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

DNA ligase 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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