We narrowed to 11,576 results for: Vars;

1. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

   Plasmid

   #114089

   Purpose

   CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized FnCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

   Plasmid

   #154263

   Purpose

   Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-ELK1

   Plasmid

   #192918

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   ELK1 (ELK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139091

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dSpCas9-NG-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223 SARS-CoV2 TEV-E

   Plasmid

   #154418

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   E (E SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of e…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   July 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PLL5.0-Anillin ShRNA-GFP-AnillinFL

   Plasmid

   #187272

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6, CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_RNF212_WT

   Plasmid

   #81860

   Purpose

   Gateway Donor vector containing RNF212 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RNF212 (RNF212 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   W4L

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

   Plasmid

   #169469

   Purpose

   Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 SARS-CoV-2 S D614-trunc

   Plasmid

   #158076

   Purpose

   Expresses SARS-CoV-2 spike protein with a truncation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_PPP6C_p.E180K

    Plasmid

    #81334

    Purpose

    Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E180K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 SARS-CoV2 TEV-M

    Plasmid

    #154419

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of m…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    July 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106195

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    GFAP

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106203

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    CAG

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS1062

    Plasmid

    #29011

    Purpose

    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple155-Cre/EGFP-NLS
    

    Use

    High copy number homologous recombination plasmid…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MiniPromoter Ple155 from the human PCP2 gene

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMS2274

    Plasmid

    #111900

    Purpose

    ssAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple344 (TUBB3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple344 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_DST-1/1

    Plasmid

    #91288

    Purpose

    Protein expression and purification of human SH3 domain construct DST-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DST-1/1 (DST Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. psiCHECK2-BRCA1 3'UTR

    Plasmid

    #29477

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    BRCA1 3'UTR (BRCA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pMXS-IRES-Blast POLD1

    Plasmid

    #160805

    Purpose

    Express POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon optimized C-terminal 230 amino acids

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_SGK1_WT

    Plasmid

    #81834

    Purpose

    Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SGK1 (SGK1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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