We narrowed to 46,691 results for: cha

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NET30 pEGFP-N2 (815)

Plasmid

#62011

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET30 (MOSPD3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
SNAP-MIDAS

Plasmid

#171604

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Tags

His6 tag and SNAP tag

Expression

Bacterial

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA6-LRRC52-V5-Flag-His

Plasmid

#127818

Purpose

Expression of human LRRC52 in mammalian cells

Depositor

Jiusheng Yan

Article

Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

Insert

LRRC52 (LRRC52 Human)

Tags

V5, FLAG, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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DTECT - Plasmid for Standard Curve

Plasmid

#139333

Purpose

This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

DTECT ligation product

Use

Unspecified

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6A (OTU, aa 128-288)

Plasmid

#61417

Purpose

Expresses human OTUD6A (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6A (OTUD6A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-127.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6B (OTU, aa 133-293)

Plasmid

#61419

Purpose

Expresses human OTUD6B (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-132.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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NET55 pEGFP-N2 (610)

Plasmid

#61992

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET55 (APH1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
p3A9cbetaTCR.20beta

Plasmid

#69592

Purpose

TCR beta chain genomic transgenic construct for creating OT-II mice

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR beta VJ region from clone 1.1 (Tcrb Mouse)

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-HOXA9

Plasmid

#131608

Purpose

expression of human HOXA9 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

HOXA9 (HOXA9 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-IBS2-7

Plasmid

#57424

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin IBS2 (Tln1 Mouse)

Tags

mEos2

Expression

Mammalian

Mutation

Talin Integrin Binding Site

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINK-OTUD2 (OTU, aa 132-314)

Plasmid

#61410

Purpose

Expresses human OTUD2 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-131 and aa 315-348.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-GST

Plasmid

#39287

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pCfB3053(gRNA X-2, XI-5, XII-4)

Plasmid

#73295

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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UASgal-A9-Pleum

Plasmid

#60343

Purpose

Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBS human Frataxin (myc tag)

Plasmid

#11443

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Tags

myc

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBabe puro human Frataxin

Plasmid

#11505

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-ODC2

Plasmid

#133257

Purpose

Express FLAG-tagged ODC2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

ODC2 (ODC2 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-NDT2

Plasmid

#133255

Purpose

Express FLAG-tagged NDT2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NDT2 (YEA6 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-Zyxin-6

Plasmid

#57441

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Zyxin (ZYX Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits