We narrowed to 11,689 results for: sas

1. pCS2+HyPer7-NES

   Plasmid

   #136467

   Purpose

   Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Nuclear export signal from the HIV Rev protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+MLS-HyPer7

   Plasmid

   #136470

   Purpose

   Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Tandem mitochondrial targeting signal of cytochro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+HyPer7-NLS

   Plasmid

   #136468

   Purpose

   Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Triple nuclear localization signal of SV40 (simia…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-GRAB_NE1m

   Plasmid

   #123308

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   contains a glycine-to-threonine mutation at posit…

   Promoter

   hSyn

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pD649-HAsp-PTK7-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156943

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PTK7 (PTK7 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-21a_3xNLS_SpCas9_protein_expression

   Plasmid

   #114365

   Purpose

   Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

   Insert

   3xNLS_SpCas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GRAB_ACh3.0

   Plasmid

   #121922

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Has Service

   AAV9

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD649-HAsp-SEMA4A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156938

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA4A (SEMA4A Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pD649-HAsp-SEMA5A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #157008

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA5A (SEMA5A Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD649-HAsp-LILRA3-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156846

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LILRA3 (LILRA3 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

    Plasmid

    #170995

    Purpose

    HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Farnselation tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+IMS-HyPer7

    Plasmid

    #136469

    Purpose

    Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    SMAC/DIABLO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD649-HAsp-ROBO2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156991

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ROBO2 (ROBO2 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD649-HAsp-ROBO1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156998

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ROBO1 (ROBO1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. AICSDP-7:SEC61B-mEGFP

    Plasmid

    #87426

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pX037

    Plasmid

    #167156

    Purpose

    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    caffeic acid cycle genes
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pNOS, p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AICSDP-4:TUBA1B-mEGFP

    Plasmid

    #87421

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. AICSDP-10:LMNB1-mEGFP

    Plasmid

    #87422

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge