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Anti-LRP4 (cytoplasmic) [N164/6.1R]

Plasmid

#114542

Purpose

Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pE-N1-RDH11

Plasmid

#161916

Purpose

Expresses untagged human RDH11. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Retinol dehydrogenase 11 (RDH11 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKD2

Plasmid

#23490

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKD2 (PRKD2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIG-727_HA-GD2-28z_CAR_RFP-tNGFR

Plasmid

#207487

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hLaminB1

Plasmid

#103800

Purpose

BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-GSK3A

Plasmid

#23510

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GSK3A (GSK3A Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIP5K1B

Plasmid

#23848

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K1B (PIP5K1B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PAK2

Plasmid

#23655

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK2 (PAK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKG2

Plasmid

#23435

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG2 (PRKG2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLKO_HA.V5.VSVg_NGFR

Plasmid

#158244

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HA.V5.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ITGB1BP3

Plasmid

#23393

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ITGB1BP3 (NMRK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-sgAMPKa2-Cas9-GFP

Plasmid

#208050

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-BRSK1

Plasmid

#23597

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRSK1 (BRSK1 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLKO_HA.V5.FLAG_NGFR

Plasmid

#158250

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HA.V5.FLAG

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-NME4

Plasmid

#23584

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME4 (NME4 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PTK2B

Plasmid

#23898

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK2B (PTK2B Human)

Use

Gateway Cloning

Mutation

K42M

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RIPK1

Plasmid

#23442

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK1 (RIPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK32C

Plasmid

#23720

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK32C (STK32C Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PTK6

Plasmid

#23899

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK6 (PTK6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIG-671_HA-GD2-28z_CAR_tNGFR

Plasmid

#207484

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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