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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,675 results for: BLI

Showing: 6081 - 6100 of 9675 results

  1. CAPZB2-TwinStrepII

    Plasmid
    #83194
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CAPZB2 (Capzb Mouse)
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83194-plasmid-map-sequence-id-163493

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  2. pLenti.puro.MCV.ER.RAZ.1

    Plasmid
    #114381
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=330 aa.
    Depositor
    Reety Arora, Sudhir Krishna
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    MCV NCCR (viral own promoter)
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114381-plasmid-map-sequence-id-221609

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  3. GAPDHS

    Plasmid
    #39010
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GAPDHS (GAPDHS Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pcDNA3.1-Beta2-PAmcherry1

    Plasmid
    #114190
    Purpose
    studying clustering of Beta2 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Insert
    Adrenergic receptor beta 2 (ADRB2 Human)
    Tags
    3HA, FLAG, and PAmcherry1
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114190-plasmid-map-sequence-id-224309

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  5. pINDUCER20-FLAG-hSRF (#799)

    Plasmid
    #78345
    Purpose
    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    SRF (SRF Human)
    Use
    Lentiviral
    Tags
    FLAG
    Promoter
    TRE2
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. 4L59

    Plasmid
    #60087
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107736
    Purpose
    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    nEF
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107736-plasmid-map-sequence-id-207278

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  9. ASH1L

    Plasmid
    #39051
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ASH1L (ASH1L Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39051-plasmid-map-sequence-id-333564

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  10. isoform PKC Delta (JCE594)

    Plasmid
    #182025
    Purpose
    yeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Delta (Prkcd Mouse)
    Expression
    Mammalian
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182025-plasmid-map-sequence-id-361958

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  11. pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

    Plasmid
    #139513
    Purpose
    B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    CAG rox stop rox td-sfGFP dre reporter
    Use
    Mouse Targeting; Dre/rox
    Expression
    Mammalian
    Available Since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    139513-plasmid-map-sequence-id-317907

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  12. pMBPcyto-TEV-BseRI

    Plasmid
    #157739
    Purpose
    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    His10 + MBP (cytoplasmic expression; no signal se…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157739-plasmid-map-sequence-id-334003

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  13. pJK372

    Plasmid
    #73597
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73597-plasmid-map-sequence-id-138754

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  14. DIRAS2

    Plasmid
    #38819
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DIRAS2 (diras2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. DAPK3

    Plasmid
    #38964
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DAPK3 (DAPK3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. 4LG7

    Plasmid
    #60094
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD4:JMC02A-C01:C228095 (MBD4 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. ALDH7A1

    Plasmid
    #38866
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH7A1 (Aldh7a1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. CD44MMA-c002

    Plasmid
    #175452
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Cd44 (Cd44 Mouse)
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175452-plasmid-map-sequence-id-353153

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  19. pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys

    Plasmid
    #90474
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(40nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    90474-plasmid-map-sequence-id-181091

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  20. pJK370

    Plasmid
    #73595
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Silent mutation in Arg299 codon.
    Promoter
    GAL1 and GAL10
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73595-plasmid-map-sequence-id-138462

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Showing: 6081 - 6100 of 9675 results