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pET16b-Trx

Plasmid

#36944

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin 1 (TXN Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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BARD1 26-327 pET28a

Plasmid

#12646

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)

Expression

Bacterial

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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3NES-CS(G)-tTA-CS(G)-3NES

Plasmid

#137836

Purpose

Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

3NES-CD(G)-tTA-CS(G)-3NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p6659 MSCV-IP N FlagHA HPV16E6

Plasmid

#44152

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
mScarlet-I-PXN

Plasmid

#112956

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

PXN

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Plasmid

#219537

Purpose

a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
NLS-tdMCP-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EMTB-mScarletI

Plasmid

#137801

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-enCjCas9-BlastR

Plasmid

#220978

Purpose

Expresses human codon-optimized enCjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

enCjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Ets2

Plasmid

#28128

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag mPTIP

Plasmid

#15557

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PTIP (Paxip1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8r-V106W

Plasmid

#208295

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-V106W-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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DHFR-TEV (integrating)

Plasmid

#247218

Purpose

Plasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Atp5-DHFR-TEV (integrating)

Plasmid

#247219

Purpose

Plasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Atp5 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Oxa1-DHFR-TEV (integrating)

Plasmid

#247220

Purpose

Plasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Oxa1 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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p15a_AcCas12n

Plasmid

#203806

Purpose

p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12n

Use

CRISPR

Promoter

rpsl

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Expression

Bacterial

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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