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pET15b+PCNA3 WT

Plasmid

#66179

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xK-T]

Plasmid

#68429

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three kink-turns

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGSTag BimEL (T112A)

Plasmid

#23101

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Alanine

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (T112D)

Plasmid

#23102

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Aspartate

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1 L5-L2

Plasmid

#62103

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D2 K112E HA

Plasmid

#8953

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Mutation

K112E: point mutation in cyclin box

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pK1_PLux*_R1_EGFP

Plasmid

#250901

Purpose

EGFP expressed under PLux* promoter; part of BNeu AS14; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Mutation

PLux* = Point mutation in PLux

Promoter

PLux*

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux*_R1_mKO2

Plasmid

#250902

Purpose

mKO2 expressed under PLux* promoter; component of BNeu AS14*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mKO2

Use

Synthetic Biology

Mutation

PLux* = Point mutation in PLux

Promoter

PLux*

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux_R1_CI*

Plasmid

#250883

Purpose

Expresses frame-shifted cI under the control of PLux promoter; part of BNeuAS2 and BNeuAS3; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame-shifted cI protein

Promoter

Plux

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-MCS-P2A-mCherry-N1

Plasmid

#225268

Purpose

Vector with a multiple cloning site, expressing a P2A ribosome skip sequence and mCherry

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

MCS-P2A-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Plasmid

#238894

Purpose

EMcapsulin class with a eUnaG fusion and an anti-mCherry intrabody (LaM-4) fusion preceded by a TGA stop codon and the rt20s readthrough motif.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(T507K)

Plasmid

#245217

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

T507K

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510L)

Plasmid

#245236

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510L

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510R)

Plasmid

#245237

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510R

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(T52S)

Plasmid

#245226

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

T52S

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Y279C)

Plasmid

#245230

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Y279C

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(E139D)

Plasmid

#245229

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

E139D

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510H)

Plasmid

#245234

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510H

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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