We narrowed to 25,266 results for: multi

1. pcDNA3.1-hFrataxin-HA

   Plasmid

   #31895

   Depositor

   Gino Cortopassi

   Article

   Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

   Insert

   Frataxin (FXN Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

2. EKARdual

   Plasmid

   #99870

   Purpose

   Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   EKAR
   

   Tags

   ShadowG and mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET11a-hRanGAP1

   Plasmid

   #50941

   Purpose

   expression of full length untagged human RanGAP1 in E.coli

   Depositor

   Frauke Melchior

   Article

   Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

   Insert

   RanGTPase activating protein 1 (RANGAP1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. AKARdual

   Plasmid

   #99869

   Purpose

   Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   AKAR4
   

   Tags

   LSSmOrange and mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6_(GLuc)_INT[3xMS2_SL]

   Plasmid

   #68426

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three MS2 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-CaMKKbeta

   Plasmid

   #32453

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221-NR1H3

   Plasmid

   #79514

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR Alpha (NR1H3 Human)
   

   Use

   Multisite gateway entry clone

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMuLE ENTR tdTomato L5-L2

   Plasmid

   #62185

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   tdTomato
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Ago1

   Plasmid

   #21534

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   Ago1 (AGO1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 13, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pJ3 SHP2 WT

    Plasmid

    #8317

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Expression

    Mammalian

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Promoter

    GAL4-responsive UAS hsp70

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-MBII85-5'SS mut

    Plasmid

    #67631

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Mutation

    mutation in 5'ss

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-SspBx6

    Plasmid

    #103778

    Purpose

    expression of iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    YFP-SspBx6
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-PKAKTRClover

    Plasmid

    #59146

    Purpose

    ORF encoding PKA KTR mClover flanked by gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA Kinase Translocation Reporter (PRKACA Human, Mouse)
    

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. G-GECO1-Orai3

    Plasmid

    #73563

    Purpose

    Green fluorescent reporter of Orai3-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    G-GECO1-Orai3 (ORAI3 Human, Synthetic)
    

    Tags

    G-GECO1

    Expression

    Mammalian

    Promoter

    CMV Immdeiate Early

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTU2S-a (p15A origin)

    Plasmid

    #74088

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. STAgR_Neo

    Plasmid

    #102992

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Promoter

    U6

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20944

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pCS2 CA-LRP6-GFP

    Plasmid

    #29682

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    LDL-receptor related protein 6 (LRP6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    extracellular region deleted

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only