We narrowed to 14,450 results for: cas9 genes

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CLTC G9.4 gRNA

Plasmid

#90634

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLTC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CLTC (Guide Designation G9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#206994

Purpose

Plasmid expressing both SpCas9 and SaCas9

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Insert

SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )

Tags

P2A

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAT009

Plasmid

#171635

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-B2M
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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DKC1 F1.3 gRNA

Plasmid

#90652

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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DKC1 F2.3 gRNA

Plasmid

#90653

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPE3-pPuro

Plasmid

#215722

Purpose

Cloning vector for Uni-Vector prime editing system

Depositor

Kou-Juey Wu

Article

Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

Insert

PE2

Use

Unspecified

Promoter

EFS promoter

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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FOXM1 C11.2 gRNA

Plasmid

#90690

Purpose

3rd generation lentiviral gRNA plasmid targeting human FOXM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FOXM1 (Guide Designation C11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1063

Plasmid

#111091

Purpose

Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

dCas9-KRAB

Use

Lentiviral

Tags

mCherry

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MP6-SlugABEN-738

Plasmid

#214348

Purpose

Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE

Expression

Bacterial

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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gh123

Plasmid

#106790

Purpose

expression of gRNA targeting ST8SIA3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA3 (ST8SIA3 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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BRCA2 A12.2 gRNA

Plasmid

#90551

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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NAE1 G8.3 gRNA

Plasmid

#90777

Purpose

3rd generation lentiviral gRNA plasmid targeting human NAE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NAE1 (Guide Designation G8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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EZH2 E9.3 gRNA

Plasmid

#90684

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1276

Plasmid

#111115

Purpose

Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

RAD51C gRNA (RAD51C Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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