We narrowed to 59,538 results for: cloning

1. pICSL50016

   Plasmid

   #50315

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
   

   Use

   Plant expression

   Mutation

   Codon-optimised for A. thaliana. BsaI/BbsI sites …

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-R4R3-3xmEGFP-C

   Plasmid

   #113751

   Purpose

   Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mEGFP-mEGFP-mEGFP
   

   Use

   CRISPR; Gateway entry vector

   Tags

   3xmEGFP

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH51

   Plasmid

   #128213

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-Kan_GY11_00131271

   Plasmid

   #121355

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   GY11_00131271
   

   Use

   Golden gate

   Mutation

   Magnaporthe oryzae

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-Kan_FR13_00004061

   Plasmid

   #121292

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   FR13_00004061
   

   Use

   Golden gate

   Mutation

   Magnaporthe oryzae

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJOG387

   Plasmid

   #105338

   Purpose

   Synthetic biology; gateway destination vector assembly

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDYSCKO

   Plasmid

   #120263

   Purpose

   Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

   Depositor

   Christian Landry

   Article

   Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

   Insert

   DYSCKO cassette
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   SNR52

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJOG545

   Plasmid

   #105424

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   AvrBs3
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH42222

   Plasmid

   #50328

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, phosphinothricin acetyl transferase, (S. hygroscopicus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation; See De…

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-R4R3-3xmRuby-N

    Plasmid

    #113754

    Purpose

    Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2-mRuby2-mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmRuby2

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUPD-ADH2t (17-21)

    Plasmid

    #101701

    Purpose

    ADH2t in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD-PGK1 (01-11)

    Plasmid

    #101689

    Purpose

    pPGK1 in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRL2697

    Plasmid

    #70693

    Purpose

    pDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinker

    Depositor

    Peter Wolk

    Article

    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

    Type

    Empty backbone

    Use

    Bacterial shuttle vector

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM4723-Del

    Plasmid

    #112207

    Purpose

    Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.755

    Type

    Empty backbone

    Use

    Golden gate assembly

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL70005

    Plasmid

    #50335

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQStrep2-PIR

    Plasmid

    #31570

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    pirin (PIR Human)
    

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOG562

    Plasmid

    #105340

    Purpose

    Synthetic biology; gateway destination vector assembly

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-Kan_GY11_00069551

    Plasmid

    #121341

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    GY11_00069551
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-Kan_GY11_00067931

    Plasmid

    #121340

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    GY11_00067931
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-Kan_GY11_00054591

    Plasmid

    #121338

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    GY11_00054591
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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