We narrowed to 15,627 results for: jun
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Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-RPS4X-3xHA plasmid
Plasmid#192246Purposeoverexpression vector of RPS4X-3XHADepositorAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-EF1a-mNeonGreen-P2A-Cas9
Plasmid#189807PurposeRetroviral delivery of Cas9DepositorInsertCas9
UseRetroviralAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187948PurposeER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertER50-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG II SIN-SV40-mCherry-SHP2
Plasmid#180800PurposeExpression of mouse SHP2 fused to N-terminal mCherryDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG II SIN-SV40-mCherry-SHP1
Plasmid#180799PurposeExpression of mouse SHP1 fused to N-terminal mCherryDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
Tags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA
Plasmid#154780Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertCRFR1 (Crhr1 Rat)
TagsHAExpressionMammalianMutation95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT sfGFP 150TAG
Plasmid#154767Purposeplasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-ICUE3-NLS
Plasmid#138255PurposeFRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertFKBP-ICUE3-NLS
TagsFKBP, Nuclear localization signal (NLS), cpVenus[…ExpressionMammalianPromoterCMVAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.3a
Plasmid#111055PurposeExpresses dLight1.3a in mammalian cellsDepositorInsertdLight1.3a
TagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
S12.1_Empty_Bridge
Plasmid#127670PurposeAmpicillin resistant multiplexing backbone without CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL437 AAVS1 EQR sgRNA
Plasmid#126447PurposeTo cut the human AAVS1 locus for integration.DepositorInsertspacer against human AAVS1 safe-harbor locus with NGAG PAM
ExpressionMammalianPromoterhuman U6Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
ExpressionBacterialMutationLysine 622 was replaced by AlanineAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.RFP657
Plasmid#69147PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
Available SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-p53(K120R)
Plasmid#72554PurposeHypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.DepositorInsertp53 (TP53 Human)
Tags5XHREExpressionMammalianMutationK120RPromoterE1B minimal promoterAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only