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pGEX4T-HEPN

Plasmid

#75296

Purpose

Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

Insert

Human Sacsin 4441-4579

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXb3

Plasmid

#73615

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXb3

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a spIgE-3xFLAG-DR18 IRES mNeonGreen-P2A-Puro

Plasmid

#248038

Purpose

Constitutively expresses 3xFLAG tagged DR18 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged DR18

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a spnull-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

Plasmid

#248033

Purpose

Constitutively expresses 3xFLAG tagged Neoleukin 2/15, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged Neoleukin-2/15

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-S214P-3xFlag

Plasmid

#245987

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-S214P mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Tags

3xFlag

Expression

Mammalian

Mutation

S214P

Promoter

CMV

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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LpABCB1-NmCherry

Plasmid

#232276

Purpose

Vector for overexpression of Lytechinus pictus ABCB1 with an N terminal mCherry tag

Depositor

Amro Hamdoun

Article

Vyas et al Development. 2022 Jun 1;149(11):dev20064

Insert

ABCB1

Use

Synthetic Biology

Tags

mCherry

Promoter

SP6

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnai3

Plasmid

#245997

Purpose

Express HA N-terminal tagged mouse Galphai3 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnai3 (Gnai3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-Y287C-3xFlag

Plasmid

#245986

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Tags

3xFlag

Expression

Mammalian

Mutation

Y287C

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnao1-B

Plasmid

#245999

Purpose

Express HA N-terminal tagged mouse GalphaoB transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnao1 (Gnao1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-3xFlag

Plasmid

#245985

Purpose

Express 3xFlag C-terminal tagged wild-type mouse GPR45 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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EF1as-ZIM3-Cas9-P2A-GFP

Plasmid

#239603

Purpose

Constitutive active CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186WT-HA

Plasmid

#239781

Purpose

Expresses WT rat NF186 and with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13198

Plasmid

#219873

Purpose

The base plasmid of TUNEYALI forTF08

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08

Expression

Yeast

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

Plasmid

#200865

Purpose

AjTn6022_TniQ expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_TnsF

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT151: pHelper(AjTn6022)_ΔTnsC

Plasmid

#200854

Purpose

Inducible expression of AjTn6022 proteins (except TnsC).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT152: pHelper(AjTn6022)_ΔTniQ

Plasmid

#200855

Purpose

Inducible expression of AjTn6022 proteins (except TniQ).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT149: pHelper(AjTn6022)_ΔTnsA

Plasmid

#200852

Purpose

Inducible expression of AjTn6022 proteins (except TnsA).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT150: pHelper(AjTn6022)_ΔTnsB

Plasmid

#200853

Purpose

Inducible expression of AjTn6022 proteins (except TnsB).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP124: pDonor(CyCAST)_0.5kb-cargo

Plasmid

#200848

Purpose

CyCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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