We narrowed to 10,237 results for: ada

1. pcDNA3-HA-DEPTOR S286/287/291A

   Plasmid

   #159160

   Purpose

   Expresses DEPTOR S286/287/291A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

   Insert

   DEPTOR (DEPTOR Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed serine 286,287 and 291 to alanine

   Promoter

   CMV

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-DEPTOR S286/287/291D

   Plasmid

   #159161

   Purpose

   Expresses DEPTOR S286/287/291D mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

   Insert

   DEPTOR (DEPTOR Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed serine 286,287 and 291 to aspartic acid

   Promoter

   CMV

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVH-EF1a-GFP-P2A-OCT4

   Plasmid

   #130692

   Purpose

   Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human OCT4 (POU5F1 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXJ42-p200 CUX1

   Plasmid

   #100813

   Purpose

   Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

   Depositor

   Alain Nepveu

   Article

   Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do

   Insert

   p200 CUX1 (CUX1 Human)
   

   Use

   Note that this plasmid does not have an sv40 ori

   Tags

   HA and Myc

   Expression

   Mammalian

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Solo-mStayGold-CENPA

   Plasmid

   #227283

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-neo-PP1-Alpha-H248K

   Plasmid

   #171268

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PPP1CA-His248Lys (H248K)

   Promoter

   CMV

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNOC_hfnCas12a_CRATES

   Plasmid

   #176251

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Integrin beta5-2XEGFP

   Plasmid

   #139779

   Purpose

   Detecting Integrin beta5 by fluorescence microscopy

   Depositor

   Staffan Stromblad

   Article

   Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

   Insert

   Integrin subunit beta5 (ITGB5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB-hCRY1-mScarlet-I-CD4-bla

   Plasmid

   #179442

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas12a-ABE7

    Plasmid

    #193644

    Purpose

    Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA-TadA*7.10, EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCh-BICD2*-strep

    Plasmid

    #120168

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    BICD cargo adaptor 2 (Bicd2 Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated BICD2: 15-595 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX304 3xFlag-eRF1 G183/184A_IRES-GFP

    Plasmid

    #198394

    Purpose

    eRF1 (AAQ mutant) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    eRF1 (ETF1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    mutated GGQ motif (G183/184A)

    Promoter

    CMV

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

    Plasmid

    #178528

    Purpose

    Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    rtTA, SspB-mScarlet-I-MYPT (1-169aa)
    

    Expression

    Mammalian

    Promoter

    CAG, Tet responsive element

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSD138A

    Plasmid

    #218130

    Purpose

    Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    luxAB
    

    Tags

    scP22

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-EMTB-TurboID-V5

    Plasmid

    #190736

    Purpose

    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    ensconsin (MAP7 Human)
    

    Tags

    Myc, TurboID, and V5

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1-dmito-oROS-HT

    Plasmid

    #216418

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    dmito-oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid

    #92140

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    AID[71-114]-eGFP
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #101061

    Purpose

    AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2-F391W
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

    Plasmid

    #159127

    Purpose

    expresses FBXO22 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    FBXO22 (FBXO22 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-Blast HA-POLE2

    Plasmid

    #160809

    Purpose

    Express HA-tagged POLE2

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    HA-POLE2 (POLE2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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