169,069 results

1. pDONR221-NPC1

   Plasmid

   #132295

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET-PE2-His (IK1822)

   Plasmid

   #170103

   Purpose

   T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

   Depositor

   Keith Joung

   Article

   Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

   Insert

   bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
   

   Use

   CRISPR

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Bacteria codon-optimized nSpCas9 (H840A) & M-…

   Promoter

   T7

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTagRFP-C1-Fibrillarin

   Plasmid

   #70649

   Purpose

   Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   TagRFP

   Expression

   Mammalian

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Rab7a-7

   Plasmid

   #55127

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab7a (RAB7A Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   N117S in NM_004637.5

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHEN2-SG4

   Plasmid

   #196071

   Purpose

   Express wild type SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4
   

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213973

   Purpose

   AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. vHB8

   Plasmid

   #193454

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   GFP-Cas9-NLS
   

   Use

   CRISPR

   Tags

   GFP

   Promoter

   GAPDH

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA 3 5' cMyc SMC1 wt

   Plasmid

   #32363

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Tags

   c-Myc

   Expression

   Mammalian

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

   Plasmid

   #205555

   Purpose

   Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRecT_2

    Plasmid

    #167546

    Purpose

    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Insert

    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    

    Expression

    Bacterial

    Promoter

    IPTG

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. VN-Tau (P301L)

    Plasmid

    #87634

    Purpose

    expresses hTau fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    MAPT (MAPT Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Mutation P301L

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQTEV- PRDX2

    Plasmid

    #31338

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PRDX2 (PRDX2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTP955

    Plasmid

    #104164

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29b-rhMOG

    Plasmid

    #225484

    Purpose

    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

    Depositor

    John Pak

    Article

    Wu et al J Immunol Methods. 2024 Oct 13:113764. d

    Insert

    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-29 (signal peptide) and 150…

    Promoter

    T7

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET32a-hEGF

    Plasmid

    #199231

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Epidermal growth factor, partial (EGF Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7 RNA POL

    Plasmid

    #138352

    Purpose

    Expressing T7 RNA Polymerase in mammalian cells

    Depositor

    Baisong Lu

    Insert

    T7 RNA POL
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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