236,394 results

1. pDESTmycDDX17

   Plasmid

   #19876

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mNeon-Blast

   Plasmid

   #207709

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-G-Flamp1

   Plasmid

   #188567

   Purpose

   green biosensor for cAMP in mammalian cells

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

   Plasmid

   #164458

   Purpose

   AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Promoter

   minCMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBC plasmid

   Plasmid

   #169746

   Purpose

   Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-BRCA1

   Plasmid

   #115491

   Purpose

   EGFP-BRCA1 expression vector. Parton clone BXY

   Depositor

   Rob Parton

   Article

   McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1009R (See depositor comments)

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV5)

   Viral Prep

   #105677-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Promoter

   Syn

   Tags

   FusionRed (Cre-dependent)

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pGP-AAV-syn-jGCaMP7b-WPRE (AAV1)

   Viral Prep

   #104489-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7b-WPRE (#104489). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7b-WPRE plasmid DNA. Syn-driven jGCaMP7b calcium sensor. jGCaMP7b exhibits the brightest resting fluorescence of the jGCaMP7 variants, and can be used for imaging of small neuronal processes. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

10. AAV-EF1a-BbChT (AAV9)

    Viral Prep

    #45186-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Promoter

    EF1a

    Tags

    mCherry, mTFP, or none

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3-GFP

    Plasmid

    #74165

    Purpose

    Mammalian expression of EGFP

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-GFP

    Plasmid

    #74165

    Purpose

    Mammalian expression of EGFP

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. A1-20B1(P004)

    Plasmid

    #184056

    Purpose

    This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

    Depositor

    C. Ron Yu

    Article

    Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

    Insert

    A1-20B1 preamplifier
    

    Use

    Synthetic Biology

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)

    Viral Prep

    #75470-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Promoter

    CAG

    Tags

    mCherry

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA-Flag-Kdm4b-pA

    Plasmid

    #104486

    Purpose

    Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

    Depositor

    Yi Zhang

    Article

    Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

    Insert

    Kdm4b (Kdm4b Mouse)
    

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Flag-Kdm4b-pA

    Plasmid

    #104486

    Purpose

    Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

    Depositor

    Yi Zhang

    Article

    Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

    Insert

    Kdm4b (Kdm4b Mouse)
    

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-N-Blast-mNeon

    Plasmid

    #207676

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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