We narrowed to 35,052 results for: URE

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pDONR-53BP1 1-1972 WT

Plasmid

#53446

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway

Mutation

siRNA resistant and mutations A128V, T720A, A1347…

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX_hDDX5_FL_wt

Plasmid

#88871

Purpose

inducible expression of GST-DDX5 in bacteria

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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mouse Oct4-GFP GOF18 transgenic reporter

Plasmid

#60527

Purpose

BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

GFPpA

Expression

Mammalian

Promoter

Oct4

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFXR1

Plasmid

#48694

Purpose

Expresses hFXR1

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FXR1 (isoform b) (FXR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pMThy1.1_hDDX5_FL

Plasmid

#88873

Purpose

constitutive expression of DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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PGL4-4X TE Down (GQs) Renilla

Plasmid

#70039

Purpose

Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

4x TE Down (G-quadruplexes) in 5'UTR

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Mutation

4 repeats of TE down motif (G-quadruplexes) in 5&…

Promoter

SV40

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCAGmCherry-gRNA

Plasmid

#87110

Purpose

gRNA cloning vector with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-HT-LC3-SV40-Hygro

Plasmid

#182045

Purpose

Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

Depositor

Hong-Gang Wang

Article

Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

HaloTag-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero45

Plasmid

#187931

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero45

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero45 (SERBP1 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-blunt-TOPO-FNDC5

Plasmid

#35970

Depositor

Bruce Spiegelman

Article

Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

Insert

fibronectin type III domain containing 5 (Fndc5 Mouse)

Use

Cloning vector

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

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pIRESh-GFPII-SM-Dlk1

Plasmid

#108932

Purpose

GFP-tagged vector expressing full length isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Tags

GFPII

Expression

Mammalian

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1_MeCP2(deltaNIC)

Plasmid

#110191

Purpose

Expression of mouse MeCP2 (deltaNIC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini. Intervening region replace…

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2

Plasmid

#72207

Purpose

Mammalian expression of full-length human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S133A

Plasmid

#46770

Purpose

Expression of GAL4 fusion to CREB-S133A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S133A (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 133 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2-YFP-CWT

Plasmid

#176689

Purpose

Fluorescent reporter for phagosomal escape of Staphylococcus aureus

Depositor

Martin Fraunholz

Article

Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

Insert

cell wall-targeting domain of Lysostaphin

Tags

YFP

Expression

Mammalian

Mutation

deletion of protase domain of Lysostaphin

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S142A

Plasmid

#46771

Purpose

Expression of GAL4 fusion to CREB-S142A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S142A (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 142 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pBKWH-p53

Plasmid

#133900

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (Trp53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only