We narrowed to 59,538 results for: cloning

1. pENTR-D-TOPO-kcnn1b

   Plasmid

   #164957

   Purpose

   Zebrafish kcnn1b gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnn1b (kcnn1b Zebrafish)
   

   Use

   Pcr cloning vector

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBALU9

   Plasmid

   #69324

   Purpose

   Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   gSL2-NLS-GFPint-FgF
   

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pX330-H11r1-2-DD-Cas9

   Plasmid

   #85978

   Purpose

   Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFOSGE_COF3H08

   Plasmid

   #70996

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Nap1 (Nap1 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMEsheP1gfp

   Plasmid

   #90130

   Purpose

   Gateway Mid-entry clone containing promoter sequence specific for She driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   sheP1Egfp (she Zebrafish)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5EsheE1

   Plasmid

   #90116

   Purpose

   5' Gateway entry clone containing endothelial specific enhancer sequence of She

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   sheE1 (she Zebrafish)
   

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBALU12

   Plasmid

   #69328

   Purpose

   Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   gSL2-NLS-mChOint-FgF
   

   Expression

   Bacterial

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMAD-Gate3

   Plasmid

   #25942

   Depositor

   Kamil Onder

   Article

   Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

   Insert

   
   

   Tags

   VP16 Fusion Protein

   Expression

   Mammalian

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS1

   Plasmid

   #55749

   Purpose

   Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH71

    Plasmid

    #128214

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

    Plasmid

    #75212

    Purpose

    Mammalian expression of SLC24A4

    Depositor

    Jonathan Lytton

    Article

    Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

    Insert

    SLC24A4 (SLC24A4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon absent

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5Ecldn5bE1

    Plasmid

    #90113

    Purpose

    5' Gateway entry clone containing endothelial specific enhancer sequence of cldn5b

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    cldn5bE1 (cldn5b Zebrafish)
    

    Expression

    Bacterial

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR_P4-P1R_5'arm_apobb.1

    Plasmid

    #128662

    Purpose

    apobb.1 homology arm 5' gateway clone

    Depositor

    Steven Farber

    Article

    Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

    Insert

    Genomic sequence upstream of apobb.1 stop codon (apobb.1 Zebrafish)
    

    Use

    Multi-step gateway cloning

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-R4R3-mEGFP-N

    Plasmid

    #113746

    Purpose

    Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    mEGFP

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-Kan_BR29_00097421

    Plasmid

    #121217

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    BR29_00097421
    

    Use

    Golden gate

    Mutation

    Magnaporthe grisea

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-Kan_CD156_AVR-Pib_70

    Plasmid

    #121286

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    CD156_AVR-Pib_70
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-Kan_CD156_ms119_98

    Plasmid

    #121285

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    CD156_AVR-Pimk1_98
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-Kan_CD156_00138051

    Plasmid

    #121284

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    CD156_00138051
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-Kan_CD156_00133411

    Plasmid

    #121283

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    CD156_00133411
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-Kan_CD156_00131711

    Plasmid

    #121282

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    CD156_00131711
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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