We narrowed to 323 results for: pcl

1. CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

   Plasmid

   #200798

   Purpose

   Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   411-680

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1.HMBP.PrS.PHF19

   Plasmid

   #125168

   Purpose

   Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   PHF19 (PHF19 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CLXSN 5myc-CDC6-WT

   Plasmid

   #109332

   Purpose

   express tagged Cdc6 WT

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Insert

   CDC6 (CDC6 Human)
   

   Use

   Retroviral

   Tags

   5x Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKrox24(MapErk)dTomato

   Plasmid

   #200113

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MapErk sequence in minimal promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   5 copies of designed MapErk sequence, no other pr…

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKrox24(2xD-E)dTomato

   Plasmid

   #200110

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-RBP-GFP(S45C)-ZdK1

   Plasmid

   #185791

   Purpose

   Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-sfGFP(S45C)-ZdK1
   

   Use

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Mutation

   Ser to Cys mutation at start of GFP, residue 45 i…

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

   Plasmid

   #200793

   Purpose

   Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   1-914; Δ505-610

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSM4

   Plasmid

   #101642

   Purpose

   Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   KCyG4219
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   C-terminal residues (221-223) deletion. Ser220Val…

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.MTF2

   Plasmid

   #125169

   Purpose

   Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   MTF2 (MTF2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.3 LRP5

    Plasmid

    #115907

    Purpose

    Expresses human LRP5 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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