We narrowed to 9,480 results for: Coli

1. pBEL2308

   Plasmid

   #181818

   Purpose

   Expression plasmid for LetB ΔRing 4 (without TM region)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetB (a.k.a. b1834, yebT, ECK1833)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta392-515)-6xHis

   Promoter

   T7

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEL2309

   Plasmid

   #181819

   Purpose

   Expression plasmid for LetB ΔRing 5 (without TM region)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetB (a.k.a. b1834, yebT, ECK1833)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta516-633)-6xHis

   Promoter

   T7

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL1749

   Plasmid

   #181820

   Purpose

   Expression plasmid for LetB ΔRing 7 (without TM region)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetB (a.k.a. b1834, yebT, ECK1833)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta747-877)-6xHis

   Promoter

   T7

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL1748

   Plasmid

   #181815

   Purpose

   Expression plasmid for LetB ΔRing 1 (without TM region)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetB (a.k.a. b1834, yebT, ECK1833)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta48-159)-6xHis

   Promoter

   T7

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEcCas5

   Plasmid

   #170090

   Purpose

   expresses E. coli type I-E Cas5

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas5
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEcCas7

   Plasmid

   #170092

   Purpose

   expresses E. coli type I-E Cas7

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas7
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEcCas8

   Plasmid

   #170093

   Purpose

   expresses E. coli type I-E Cas8

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEcCse2

   Plasmid

   #170094

   Purpose

   expresses E. coli type I-E Cse2

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cse2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYCDuet_DL121_WTTS

   Plasmid

   #171954

   Purpose

   Selection vector for chimeric fusion of ecDHFR and asLOV2.

   Depositor

   Kimberly Reynolds

   Article

   McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

   Inserts

   Fusion of ecDHFR and asLOV2 at position 121 of DHFR
   Thymidylate Synthase
   

   Use

   Tags

   8-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW228

    Plasmid

    #113242

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->tAggtag

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW227

    Plasmid

    #113241

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->Atggtag

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW217

    Plasmid

    #113239

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtaC

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW213

    Plasmid

    #113235

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->Cgtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAW211

    Plasmid

    #113233

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->AACGA

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAW216

    Plasmid

    #113238

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtTg

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCASCADE-gltA1-gltA2

    Plasmid

    #71348

    Purpose

    gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    gltA1-gltA2 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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