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We narrowed to 11,131 results for: Hal

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pHAGE-EFS-MCP-HALOnls

Plasmid

#121937

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

MCP-HALOnls

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209722

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209651

Purpose

Expression of iATPSnFR2, high affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Tags

Expression

Mutation

S29W.A95K

Promoter

CAG

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-mEGFP-reHaloTagF

Plasmid

#200190

Purpose

Tom20 with reversible HaloTagF variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Use

Tags

mEGFP and reHaloTagF

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET302-6His-dCas9-Halo

Plasmid

#72269

Purpose

Encodes a Halo-labeled fusion of nuclease deficient Cas9

Depositor

Timothée Lionnet

Article

Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

Insert

His6-dCas9-Halo

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-pSu9-HaloTag7-mGFP

Plasmid

#184905

Purpose

Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

Presequence of Fo-ATPase subunit 9

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209664

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

hSyn

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag7

Plasmid

#175533

Purpose

Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7

Plasmid

#167266

Purpose

Overexpression of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Use

Tags

His x10

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP-KDEL

Plasmid

#184904

Purpose

Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

KDEL

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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HaloTag-beta2-adrenergic receptor

Plasmid

#66994

Purpose

Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-beta2-adrenergic receptor (ADRB2 Human)

Use

Tags

Human Growth hormone Signal Sequence-HaloTag

Expression

Mammalian

Mutation

The signal sequence from human growth hormone was…

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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Halo-Tev-Flag-Ube2v2

Plasmid

#110070

Purpose

Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

Depositor

Yimon Aye

Article

Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

Insert

Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)

Use

Tags

Halo-TEV-Flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-HaloCaMP1a-EGFP

Plasmid

#138327

Purpose

chemigenetic calcium indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Has Service

AAV1 and AAV9

Insert

HaloCaMP1a

Use

AAV

Tags

EGFP, His-tag, and Nuclear Exclusion Signal

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag141/145

Plasmid

#194660

Purpose

Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag141/145

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag154/156

Plasmid

#194661

Purpose

Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag154/156

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209652

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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HaloTag-bActinCDS-bActinUTR-MS2V5

Plasmid

#102718

Purpose

Halo-Actin reporter for detection of both mRNA and protein

Depositor

Robert Singer

Article

Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

HaloTag-bActinCDS-bActin3'UTR-MS2V5

Use

Lentiviral

Tags

HaloTag and MS2V5

Expression

Mutation

Promoter

UbC

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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