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Plasmid#126689PurposeContains Blue Fluorescent Protein insert and a lineage-specific human surfactant protein C (SPC) promoter driving BFP expression.DepositorInsertTagBFP
UseLentiviralTagsExpressionMutationPromoterhSPCAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-RSV-jGCaMP7s-W
Plasmid#126690PurposeMammalian calcium sensor protein.DepositorInsertjGCaMP7s
UseLentiviralTags6xHIS, T7, XpressExpressionMutationA52V, A317LPromoterRSVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-RSV-jGCaMP7f-W
Plasmid#126691PurposeMammalian calcium sensor protein.DepositorInsertjGCaMP7f
UseLentiviralTags6xHIS, T7, XpressExpressionMutationH78K, A317LPromoterRSVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>GAL80
Plasmid#104874PurposeHACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.DepositorInsertT2A-GAL80
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-hSPC-TFEBeGFP-W
Plasmid#126692PurposeLineage specific over expression of TFEB in lung alveolar epithelial cellsDepositorInsertTFEB (TFEB Human)
UseLentiviralTagsExpressionMutationDeleted Stop Codon on TFEBPromoterhSPCAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-hSPC-TFEB(delta30)eGFP-W
Plasmid#126693PurposeLineage specific over expression of a mutated TFEB in lung alveolar epithelial cells, where TFEB is missing the first 30 amino acids of the sequence.DepositorInsertTFEB(delta30) (TFEB Human)
UseLentiviralTagsExpressionMutationDeleted amino acids 1-30, H32D, Deleted Stop Codo…PromoterhSPCAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-empty chr I
Plasmid#41895DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPDAvailable sinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
UseTagsExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAC-ECFP-15xQUAS_TATA-SV40
Plasmid#104875PurposepiggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-NT*-HRV3CP
Plasmid#162795PurposeExpressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)DepositorInsertHuman rhinovirus 14 3C protease
UseTagsNT* solubility tagExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
UseTagsExpressionBacterialMutationPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR1K18ms
Plasmid#72644PurposeGateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringaeDepositorTypeEmpty backboneUseSucrose counter-selection allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceMarch 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAC-DsRed-QF2-hsp70
Plasmid#104876PurposeGeneral purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquitoDepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-nlsGFP-W
Plasmid#126688PurposeConstitutive, ubiquitous, nuclear-localized GFP expression.DepositorInsertnlsGFP
UseLentiviralTags6xHIS and NLSExpressionMutationPromoterEF1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAC-ECFP-15xQUAS_TATA-mcd8-GFP-SV40
Plasmid#104878PurposepiggyBac vector of 15xQUAS-mCD8:GFP; used for mosquito tranformationDepositorInsertmCD8:GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-AVT1 chr I
Plasmid#41898PurposeYeast expression of AVT1.DepositorInsertAVT1 (AVT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBac-DsRed-ORCO_9kbProm-QF2
Plasmid#104877PurposeAnopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoesDepositorInsertAgORCO-QF2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST1K18ms
Plasmid#72646PurposeGateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringaeDepositorTypeEmpty backboneUseSucrose counter-selection allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceMarch 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pluc2-iRFP720
Plasmid#98887PurposeMammalian expressionDepositorInsertFirefly Luciferase
UseTagsiRFP720ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only